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Expression of cellular-yes protein in mammalian tissues

Y H Zhao1, J G Krueger, M Sudol

  • 1Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021.

Oncogene
|November 1, 1990
PubMed

Insights

The c-yes proto-oncogene, p62c-yes, is expressed in various mammalian tissues, with high levels found in epithelial cells, platelets, and spermatid acrosomes. Its varied expression suggests involvement in diverse cellular pathways.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • The c-yes proto-oncogene encodes a non-receptor tyrosine kinase.
  • Understanding the expression patterns of proto-oncogenes is crucial for comprehending normal cellular functions and disease development.

Purpose of the Study:

  • To investigate the expression and localization of the c-yes proto-oncogene (p62c-yes) in normal mammalian tissues.
  • To determine the differential expression levels and intracellular distribution of p62c-yes across various cell types and organs.

Main Methods:

  • Immunohistochemistry was employed to visualize p62c-yes expression in tissue sections.
  • Immunoprecipitation assays using affinity-purified anti-yes IgG were performed to detect and quantify p62c-yes.
  • An antibody was generated against a bacterial fusion protein of the human c-yes amino-terminal domain.

Main Results:

  • p62c-yes was detected in a wide range of rat and human tissues.
  • Significant variations in p62c-yes expression levels were observed among different cell types.
  • Highest expression levels were noted in certain epithelial cells, platelets, and spermatid acrosomes.
  • Intracellular localization of p62c-yes in epithelial cells varied depending on the organ's physiological function.

Conclusions:

  • The expression pattern of p62c-yes is widespread yet cell-type specific in normal mammalian tissues.
  • The diverse localization and expression levels suggest a role for p62c-yes in multiple cellular processes and pathways.
  • Further research into p62c-yes function is warranted based on its broad expression profile.

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