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Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
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Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function

D J DiLillo1, J B Weinberg, A Yoshizaki

  • 1Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.

Leukemia
|June 21, 2012
PubMed

Insights

Chronic lymphocytic leukemia (CLL) cells produce interleukin-10 (IL-10), similar to regulatory B10 cells. These IL-10-producing CLL cells may contribute to the immunosuppression seen in patients.

Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • Chronic lymphocytic leukemia (CLL) is associated with immunosuppression.
  • CLL cells share markers with regulatory B cells (B10 cells) that produce interleukin-10 (IL-10).

Purpose of the Study:

  • To investigate the functional links between CLL cells and regulatory B10 cells.
  • To assess the IL-10 production capacity of leukemia cells in CLL patients.

Main Methods:

  • Phenotypic analysis and IL-10 expression assessment of leukemia cells from 93 CLL patients.
  • Evaluation of IL-10 levels in patient serum.
  • Analysis of IL-10-competent B cells in TCL1-transgenic mouse models of CLL.

Main Results:

  • CD5(+) CLL cells from 90% of patients were IL-10-competent and secreted IL-10 upon stimulation.
  • Elevated serum IL-10 levels were observed in CLL patients.
  • IL-10-competent B cells expanded in TCL1-Tg mice, preceding leukemia development, and malignant CLL cells exhibited regulatory functions.

Conclusions:

  • Malignant CLL cells are IL-10-competent and possess regulatory functions akin to B10 cells.
  • IL-10 production by CLL cells may contribute to immunosuppression in CLL patients and mouse models.

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