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Updated: May 15, 2026
Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
Published on: March 25, 2020
Thy-1 knockdown retards wound repair in mouse skin
Min-Jung Lee1, Jeong-Oh Shin, Han-Sung Jung
1Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Republic of Korea.
Insights
Blocking Thy-1 (CD90) in skin wounds impairs healing and slows repair. This leads to increased TGF-β1 levels and affects re-epithelialization, highlighting Thy-1
Area of Science:
- Dermatology
- Cell Biology
- Wound Healing Research
Background:
- Thy-1 (CD90) is a cell surface glycoprotein found in lipid raft microdomains.
- Its function in skin wound healing is not well understood.
Purpose of the Study:
- To investigate the role of Thy-1 in skin wound healing in vivo.
- To determine the effects of blocking Thy-1 on the repair process.
Main Methods:
- Utilized Thy-1 siRNA to temporarily block Thy-1 expression in skin wound regions.
- Compared wound healing in vivo with and without Thy-1 siRNA treatment.
Main Results:
- Blocking Thy-1 significantly worsened the quality and rate of skin wound healing.
- Thy-1 inhibition led to a continuous increase in TGF-β1 levels at the wound site.
- Thy-1 expression was primarily observed in the wound dermis.
Conclusions:
- Blocking Thy-1 in wound areas reduces the efficiency of skin repair.
- Thy-1 inhibition negatively impacts re-epithelialization and promotes TGF-β1 over-expression during skin healing.
Background:
Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains.
Objective:
The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear.
Methods And Results:
In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased.
Conclusion:
These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.
