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Updated: Aug 11, 2026

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
11:14

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Published on: October 12, 2018

[The lymphocyte populations at different stages in the infection caused by the human immunodeficiency virus]

V V Pokrovskiĭ, D I Gabrilovich, L V Serebrovskaia

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |February 1, 1990
    PubMed

    Insights

    This study on HIV infection found increased rosette-forming cells (RFCs) in asymptomatic individuals. Later stages showed elevated T8 lymphocytes and a decreased T4/T8 ratio, with T4 cells dropping sharply in AIDS patients.

    Area of Science:

    • Immunology
    • Virology
    • Cell Biology

    Background:

    • Human Immunodeficiency Virus (HIV) infection significantly impacts the immune system.
    • Understanding changes in immunocompetent cells is crucial for monitoring disease progression.

    Purpose of the Study:

    • To comparatively analyze immunocompetent cell content in HIV-infected individuals.
    • To correlate cell changes with different stages of HIV infection.

    Main Methods:

    • Utilized rosette formation and immunofluorescence assays.
    • Employed monoclonal antibodies for cell identification.
    • Studied 58 individuals across various HIV infection stages.

    Main Results:

    • Asymptomatic HIV infection showed increased rosette-forming cells (RFCs), including active E-RFC, E-RFC, theophylline-resistant E-RFC, and M-FRC.
    • Lymphadenopathy stage revealed elevated T8 lymphocytes, particularly with secondary infections, and the first decrease in the T4/T8 ratio.
    • Advanced HIV/AIDS stages maintained high RFC levels but exhibited a sharp decline in T4 lymphocytes.

    Conclusions:

    • RFC levels may serve as an indicator in early HIV stages.
    • T8 lymphocyte increase and T4/T8 ratio decrease are characteristic of later HIV progression.
    • Significant T4 lymphocyte depletion marks the development of AIDS.

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