Related Experiment Videos
Polarization and capping of lymphocytes in HIV infection
D Torre1, A Pugliese, C Lamperti
1Division of Infectious Diseases, Regional Hospital, Varese, Italy.
Insights
Human immunodeficiency virus (HIV) infection significantly impairs lymphocyte locomotor capacity in patients with acquired immunodeficiency syndrome (AIDS). While lymphocyte capping was reduced, the effect was not statistically significant in this study.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Human immunodeficiency virus (HIV) infection affects immune cells, including lymphocytes.
- Lymphocyte function, such as motility and surface receptor distribution, can be altered during HIV infection.
Purpose of the Study:
- To evaluate the impact of HIV infection on lymphocyte locomotor capacity using a polarization assay.
- To assess the capping phenomenon of lymphocytes in HIV-infected patients and in vitro with HIV envelope glycoproteins.
Main Methods:
- Lymphocyte polarization assay was employed to measure locomotor capacity.
- Lymphocyte capping was evaluated in acquired immunodeficiency syndrome (AIDS) patients and in vitro with gp41 and gp120 glycoproteins.
Main Results:
- A significant reduction in polarized lymphocytes was observed in patients with acquired immunodeficiency syndrome (AIDS).
- Lymphocyte capping was reduced in AIDS patients and in vitro exposed cells, but this reduction was not statistically significant.
Conclusions:
- HIV infection, particularly in AIDS patients, leads to a significant decrease in lymphocyte locomotor capacity.
- While HIV envelope glycoproteins may influence lymphocyte capping, the effect requires further investigation to reach statistical significance.
Abstract:
The present study was undertaken to assess the locomotor capacity of lymphocytes from patients with human immunodeficiency virus (HIV) infection by a polarization assay. In addition, the capping phenomenon of lymphocytes from HIV-infected patients or in vitro preincubation of lymphocytes with HIV-envelope glycoproteins, gp41 and gp120 was evaluated. A significant decrease in polarized lymphocytes from patients with acquired immunodeficiency syndrome (AIDS) was observed. On the other hand, the capping phenomenon either of lymphocytes from AIDS patients or of lymphocytes from healthy donors preincubated with gp41 and gp120 was reduced but not significantly.