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Identification of a 43-kDa nuclear antigen associated with proliferation by monoclonal antibody K 112
J J Quak1, G van Dongen, M A Koken
1Dept. of Otolaryngology/Head and Neck Surgery, Free University Hospital, Amsterdam, The Netherlands.
Insights
Monoclonal antibody K 112 identifies a nuclear antigen present only in cycling cells. This cell-cycle-related protein is found across mammalian species, offering potential for cell proliferation research.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- Squamous-cell carcinoma research often involves identifying specific cellular markers.
- Understanding cell cycle regulation is crucial for cancer biology.
Purpose of the Study:
- To generate and characterize a novel monoclonal antibody (MAb) for identifying cell-cycle-specific antigens.
- To investigate the expression patterns and species reactivity of the MAb K 112 target antigen.
Main Methods:
- Intrasplenic immunization of a mouse with laryngeal squamous-cell carcinoma.
- Production and characterization of monoclonal antibody K 112.
- Immunodetection of the target antigen in various cell types and tissues.
Main Results:
- MAb K 112 successfully detected a 43-kDa nuclear antigen with a pI of 5.4.
- The antigen is exclusively expressed in cycling cells, with distinct localization during mitosis.
- The antigen showed conserved reactivity across all tested mammalian species.
Conclusions:
- MAb K 112 recognizes a conserved, cell-cycle-dependent nuclear antigen.
- This antigen is a potential marker for proliferating cells in mammalian systems.
- The findings support the utility of MAb K 112 in cell cycle studies and cancer research.
Abstract:
Monoclonal antibody (MAb) K 112 was generated after a single intrasplenic immunization with a recurrent laryngeal squamous-cell carcinoma. The antibody detects a 43-kDa nuclear antigen, with a pI of 5.4, which is expressed only in cycling cells. Expression is typically seen in a granular pattern excluding the nucleoli. During mitosis the bulk of the antigen is diffusely distributed in the cytoplasm. Identical reactivity was observed for tissues or cells of all mammalian species tested. These data indicate that MAb K 112 recognizes a protein belonging to the class of cell-cycle-related nuclear antigen molecules.