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Label-free impedimetric immunosensor for sensitive detection of fenvalerate in tea
Meirong Wang1, Huimin Kang, Dan Xu
1College of Chemistry and Chemical Engineering, Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou University, 180 Si-Wang-Ting Road, Yangzhou 225002, China.
Insights
A new immunosensor detects fenvalerate pesticide with high sensitivity and a wide linear range. This method offers a simple, stable, and reproducible way to analyze real samples like tea.
Area of Science:
- Electrochemistry
- Biosensors
- Analytical Chemistry
Background:
- Fenvalerate is a widely used synthetic pyrethroid insecticide.
- Accurate detection of fenvalerate is crucial for food safety and environmental monitoring.
- Existing detection methods can be complex or lack sensitivity.
Purpose of the Study:
- To develop a novel electrochemical immunosensor for fenvalerate detection.
- To optimize the sensor's performance for enhanced sensitivity and reliability.
- To validate the sensor's applicability in real-world sample analysis.
Main Methods:
- Immobilization of fenvalerate antibodies onto a chitosan-modified glassy carbon electrode (GCE).
- Utilizing electron transfer resistance changes with a Fe(CN)6(3-)/Fe(CN)6(4-) probe to quantify fenvalerate.
- Optimization of reaction conditions for sensor performance.
Main Results:
- A linear relationship was observed between resistance change and fenvalerate concentration (logarithmic scale) from 1.0×10(-3) to 1.0×10(1) mg/L (R=0.998).
- A low detection limit of 0.80 μg/L was achieved.
- The immunosensor demonstrated simplicity, high sensitivity, specificity, reproducibility, and good stability.
Conclusions:
- The developed immunosensor provides a sensitive and reliable method for fenvalerate detection.
- The sensor successfully detected fenvalerate in real tea samples.
- The immunosensor is regenerable, allowing for repeated use.
Abstract:
In this experiment, fenvalerate antibodies were immobilised on the electrode by the crosslinking with glutaraldehyde modified on the glassy carbon electrode (GCE) via chitosan. Fenvalerate was measured by the increase of electron transfer resistance when the immune reaction occurred with Fe(CN)6(3-)/Fe(CN)6(4-) as the probe. Under optimal conditions, the change of resistance is in a linear relationship with the logarithm of the concentration in the range of 1.0×10(-3)∼1.0×10(1)mg/L (R=0.998) with a detection limit of 0.80 μg/L. This method bears such merits as simplicity of operation, high sensitivity, wide linear range, specificity, reproducibility and good stability. The immunosensor was applied in the detection of real samples of tea, achieving satisfactory results, and it could be regenerated after being placed alternately in 0.5 mol/L HCl and 0.5 mol/L NaOH solutions.
