Related Experiment Videos
In vivo localization of antitumor lymphocytes separated with Dolichos biflorus lectin
1Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Insights
Murine splenocytes not agglutinable with Dolichos biflorus agglutinin (DBA) preferentially localize to the spleen. This splenic accumulation, particularly in the white pulp, contributes to their potent antitumor activity.
Area of Science:
- Immunology
- Cell Biology
- Cancer Research
Background:
- Dolichos biflorus agglutinin (DBA) agglutination separates murine splenocytes.
- DBA- cells exhibit high antitumor efficacy.
- Understanding the in vivo localization of DBA- cells is crucial.
Purpose of the Study:
- To elucidate the in vivo localization mechanism of DBA- murine splenocytes.
- To correlate splenocyte localization with antitumor efficacy.
Main Methods:
- In vivo intravenous injection of 51Cr-labeled and [3H]TdR-labeled DBA-separated splenocytes into mice.
- Gamma counting of tissue samples for cell localization.
- Autoradiography of spleen sections to determine precise accumulation sites.
Main Results:
- DBA- cells showed significant localization in the spleen compared to DBA+ cells.
- Autoradiography revealed concentration of DBA- cells in the spleen's white pulp.
- These findings suggest active recirculation of DBA- cells.
Conclusions:
- DBA- splenocytes accumulate preferentially in the spleen, particularly the white pulp.
- Splenic localization and active recirculation contribute to the potent in vivo antitumor activity of DBA- cells.
Abstract:
We examined the in vivo localization of Con A sup-cultured murine splenocytes separated with Dolichos biflorus agglutin (DBA) to elucidate the mechanism underlying the high antitumor efficacy of cells which are not agglutinable with DBA (DBA- cells). First, 51Cr-labeled DBA-separated cells were injected intravenously (i.v.) into mice and then the tissues were collected for gamma counting. DBA- cells showed significant localization in the spleen, compared with DBA+ cells. This was further confirmed by injecting Thy1.2-positive DBA- cells into Thy1.1 congenic mice. Furthermore, after [3H]TdR-labeled DBA-separated cells had been injected i.v., the spleens of the recipients were fixed, sectioned and processed for autoradiography to determine in which region of the spleen the labeled cells had accumulated. We found that DBA- cells were especially concentrated in the white pulp of the spleen, where lymphocytes are localized before being recirculated. These results suggest that DBA- cells exert strong antitumor activity in vivo partly due to their localization in the spleen and partly to the high incidence of active recirculation.