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Phytomitogen induced changes in levels of inositol phosphates in the bovine lymphocytes

Jyotsna Behl nee Dhingra1, N K Verma, Rahul Behl

  • 1National Bureau of Animal Genetic Resources, P. Box No. 129, Karnal 132 001, India. jyotsna@nbagr.ernet.in

Indian Journal of Biochemistry & Biophysics
|August 9, 2013
PubMed

Insights

Bovine lymphocytes rapidly increase inositol phosphates when stimulated by phytomitogens like concanavalin A and phytohaemagglutinin. This response involves a quick turnover of phosphoinositides, particularly inositol diphosphate.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Inositol phosphates and phosphoinositides are crucial signaling molecules in cellular processes.
  • Phytomitogens, such as concanavalin A (Con A) and phytohaemagglutinin (PHA), are known to activate lymphocytes.

Purpose of the Study:

  • To investigate the dynamic changes in inositol phosphates and phosphoinositides in bovine lymphocytes upon stimulation with Con A and PHA.
  • To elucidate the specific inositol phosphate fractions involved in the early cellular response.

Main Methods:

  • Bovine lymphocytes were stimulated with concanavalin A (Con A) and phytohaemagglutinin (PHA).
  • Levels of inositol phosphates and phosphoinositides were quantified using cpm measurements at 1 and 5 minutes post-stimulation.

Main Results:

  • Con A and PHA induced a rapid increase in total inositol phosphates within 1 minute.
  • A concurrent decrease in phosphatidylinositol levels was observed.
  • Inositol diphosphate showed the most significant increase, contributing to the overall rise in total inositol phosphates.

Conclusions:

  • Bovine lymphocytes exhibit a swift phosphoinositide turnover in response to phytomitogen stimulation.
  • The findings highlight the role of inositol phosphate signaling in lymphocyte activation.

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