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Updated: May 9, 2026
A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
Published on: January 5, 2021
Chinese goose (Anser cygnoides) CD8a: cloning, tissue distribution and immunobiological in splenic mononuclear cells
Qiurong Zhao1, Fei Liu, Shun Chen
1Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46 Xinkang Road, Ya'an, Sichuan 625014, PR China.
Insights
Researchers identified the Chinese goose CD8α (goCD8α) gene, crucial for cell-mediated immunity. Its expression varied in immune organs and increased in response to viral infection and specific immune stimulation.
Area of Science:
- Immunology
- Genetics
- Avian Biology
Background:
- The CD8 molecule is a vital cell membrane glycoprotein in cell-mediated immunity.
- Understanding avian CD8α provides insights into immune responses in poultry.
Purpose of the Study:
- To identify and characterize the Chinese goose CD8α (goCD8α) gene.
- To investigate the expression patterns of goCD8α in different tissues and under various conditions.
Main Methods:
- Gene identification and sequencing of full-length cDNA.
- Phylogenetic analysis of waterfowl CD8α.
- Semi-quantitative RT-PCR to analyze mRNA expression levels.
Main Results:
- The full-length goCD8α cDNA is 1459bp with a 711bp open reading frame.
- Phylogenetic analysis confirmed waterfowl CD8α form a monophyletic group.
- goCD8α mRNA was highly expressed in immune organs and mucosal tissues in goslings, with notable increases in spleen during acute viral enteritis and in thymus, bursa of fabricius, and cecum during chronic infection.
Conclusions:
- The goCD8α gene has been identified and characterized in Chinese geese.
- goCD8α expression is tissue-specific and responsive to viral infections and immune stimuli, highlighting its role in goose immunity.
Abstract:
CD8 molecule is a cell membrane glycoprotein, which plays an important role in cell-mediated immunity. Here, we identified Chinese goose CD8α (goCD8α) gene for the first time. The full-length cDNA of goCD8α is 1459bp in length and contains a 711bp open reading frame. Phylogenetic analysis shows that the waterfowl CD8α formed a monophyletic group. Semi-quantitative RT-PCR analysis showed that transcripts of goCD8α mRNA were high in the immune-related organs and mucosal immune system in gosling, and high in thymus and spleen comparing to other immune-related tissues in goose. The obvious increase of CD8α expression was observed in spleen of acute new type gosling viral enteritis virus (NGVEV) infected bird, while the increase of CD8α were observed in the thymus, bursa of fabricius, and cecum of chronic infected bird. The CD8α mRNA transcription level in spleen mononuclear cells was significantly up-regulated when stimulated by phytohemagglutinin, but not by lipopolysaccharide in vitro.
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