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Proteomic approach for identification of immunogenic proteins of Mycoplasma mycoides subsp. capri
1Istituto Zooprofilattico Sperimentale della Sardegna "G. Pegreffi", 07100 Sassari, Italy.
Insights
Researchers identified key proteins from Mycoplasma mycoides subsp. capri using an immunoproteomic approach. These immunogenic proteins show potential as biomarkers for diagnosing contagious agalactia in goats.
Area of Science:
- Veterinary Microbiology
- Proteomics
- Immunology
Background:
- Mycoplasma mycoides subsp. capri is a significant pathogen causing contagious agalactia in goats.
- Accurate serological diagnosis is crucial for controlling and eradicating the disease.
Purpose of the Study:
- To identify immunodominant proteins of Mycoplasma mycoides subsp. capri using an immunoproteomic strategy.
- To evaluate the potential of these identified proteins as diagnostic biomarkers for contagious agalactia.
Main Methods:
- An immunoproteomic approach combining TX-114 phase partitioning, 2D electrophoresis, Western blotting, and nanoLC-ESI-MSMS was employed.
- Membrane proteins from Mycoplasma mycoides subsp. capri isolates were analyzed using pooled sera from naturally infected goats.
Main Results:
- A total of 13 distinct immunogenic proteins were identified from Mycoplasma mycoides subsp. capri.
- These proteins are primarily metabolic enzymes involved in carbohydrate and energy metabolism.
- Notable identified proteins include pyruvate dehydrogenase, translation elongation factors, and trigger factor.
Conclusions:
- The identified immunogenic proteins are potential candidates for developing specific and sensitive biomarkers for the serological diagnosis of contagious agalactia.
- This finding could lead to improved diagnostic tools for Mycoplasma mycoides subsp. capri infections.
Abstract:
In this study, an immunoproteomic approach was used to identify immunodominant proteins from Mycoplasma mycoides subsp. capri isolates. Membrane proteins, extracted through TX-114 phase partitioning, were separated using mono- and two-dimensional electrophoresis and detected by Western blotting with pooled sera from naturally infected goats. A total of 27 immunoreactive spots, corresponding to 13 different proteins, were identified using nanoLC-ESI-MSMS. Function annotation revealed that most of these proteins were metabolic enzymes involved in carbohydrate and energy metabolism. The immunogenic proteins identified in this study: pyruvate dehydrogenase, dihydrolipoamide acetyltransferase, dihydrolipoyl dehydrogenase, phosphate acetyltransferase, phosphopyruvate hydratase, adenine phopshoribosyltransferase, transketolase, translation elongation factor G, translation elongation factor Ts, FMN-dependent NADH-azoreductase, peptide methionine sulfoxide reductase, inorganic diphosphatase and trigger factor may be used as biomarkers for the serological diagnosis of contagious agalactia caused by M. mycoides subsp. capri.
