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Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry

Kirstie Canene-Adams1

  • 1Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Methods in Enzymology
|November 5, 2013
PubMed

Insights

This protocol details tissue preservation for research. Formalin fixation and paraffin embedding prepare animal and human biopsy or whole organ samples for future immunohistochemistry experiments.

Area of Science:

  • Histopathology
  • Biotechnology
  • Research Methodology

Background:

  • Standardized tissue preservation is crucial for reliable experimental outcomes.
  • Formalin fixation and paraffin embedding (FFPE) are widely used techniques in histopathology.
  • Accurate sample preparation is essential for subsequent molecular and cellular analyses.

Purpose of the Study:

  • To provide a detailed protocol for formalin fixation and paraffin embedding of animal and human tissues.
  • To ensure tissue integrity and morphology for immunohistochemistry (IHC) applications.
  • To serve as a foundational method for various downstream tissue-based research.

Main Methods:

  • Obtain biopsy or whole organ tissue specimens from animal or human subjects.
  • Immerse tissues in formalin for fixation to preserve cellular structure.
  • Embed the fixed tissues into paraffin blocks for long-term storage and sectioning.

Main Results:

  • Successfully preserved tissue architecture and cellular details.
  • Prepared specimens suitable for immunohistochemistry.
  • Ensured sample stability for future experimental use.

Conclusions:

  • This protocol effectively prepares tissue samples for advanced research techniques like IHC.
  • The method ensures high-quality tissue preservation for both animal and human samples.
  • Adherence to this protocol supports reproducible and reliable scientific investigations.

Keywords:
Fixation of tissueFormalin-fixed paraffin-embedded tissue (FFPET)ImmunohistochemistryTissue process and embeddedTissue section

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