Immunosorbent assay using gold colloid cluster technology for determination of IgEs in patients' sera

Area of Science:

• Biotechnology
• Immunochemistry
• Analytical Chemistry

Background:

• Traditional immunoassays like ELISA can be time-consuming and require specialized equipment.
• A need exists for simpler, faster, and equally sensitive methods for detecting antigens and antibodies.

Purpose of the Study:

• To develop and validate a Cluster-Linked Immunosorbent Assay (CLISA) utilizing gold colloidal cluster (GCC) labeling.
• To establish CLISA as a sensitive, simple, and rapid method for detecting protein interactions, serving as an alternative to existing assays.

Main Methods:

• Antibodies were labeled with gold colloidal clusters (GCCs) via their Fc domain.
• The GCC-labeled antibodies' Fab domain was used to detect target antigens dotted on nitrocellulose membranes or microtiter plates.
• Visual detection and quantification using a color scale from known concentrations were employed.

Main Results:

• The CLISA method demonstrated sensitive detection of antigen-antibody interactions, including β-lactoglobulin.
• The colored reaction product was stable and visible to the naked eye, allowing for easy interpretation.
• The assay proved effective for detecting IgEs in patient sera, showcasing its analytical utility.

Conclusions:

• CLISA is a simple, fast, and convenient method for detecting antigen or antibody biorecognitions.
• The assay offers a stable and visually detectable signal, making it a viable alternative to enzyme-linked immunosorbent assays.
• The developed CLISA shows promise for various analytical applications, including clinical diagnostics.