Coffea arabica Seed Extract Stimulate the Cellular Immune Function and Cyclophosphamide-induced Immunosuppression in...

Area of Science:

• Immunology
• Pharmacology
• Natural Product Research

Background:

• Cyclophosphamide (CP) is a chemotherapeutic agent known to suppress both cellular and humoral immunity.
• Assessing the immunostimulatory potential of natural products is crucial for developing novel immunomodulatory agents.
• Coffee seeds (Coffea arabica) are rich in bioactive compounds with potential health benefits.

Purpose of the Study:

• To investigate the immunostimulatory effects of alcoholic coffee seed extract.
• To evaluate the extract's efficacy in mitigating cyclophosphamide-induced immunosuppression.
• To assess the impact on cell-mediated and humoral immune responses.

Main Methods:

• Mice were treated with varying doses of coffee seed extract (50, 150, 250 mg/Kg).
• Cell-mediated immunity was assessed using the delayed-type hypersensitivity (DTH) response.
• Humoral immunity was evaluated by hemagglutination antibody titre (HT).
• Relative organ weights (spleen, thymus, kidney, liver) and white blood cell (WBC) counts were analyzed.

Main Results:

• Coffee seed extract significantly increased relative spleen and thymus weights at 50 and 150 mg/Kg doses.
• WBC counts were significantly elevated across all extract doses.
• A significant increase in DTH response was observed at 50 and 150 mg/Kg, indicating enhanced cellular immunity.
• The extract demonstrated a modulatory effect on humoral immunity in the HT test.

Conclusions:

• Alcoholic coffee seed extract exhibits significant immunostimulatory properties.
• The extract effectively counteracts cyclophosphamide-induced immunosuppression in mice.
• Coffee seed extract shows potential as a natural agent for immune system enhancement.