Related Experiment Video

Updated: May 4, 2026

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
07:35

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Published on: July 5, 2017

14.2K

Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue

Sebastian Siebelmann1, Uta Gehlsen1, Gereon Hüttmann2

  • 1Department of Ophthalmology, University of Cologne, Cologne, Germany.

Plos One
|December 31, 2013
PubMed

Insights

Conjunctiva-associated lymphoid tissue (CALT) in mice shows dynamic changes in development and cellular activity. This research provides insights into CALT

Area of Science:

  • Immunology
  • Ocular Surface Research
  • Microscopy

Background:

  • Conjunctiva-associated lymphoid tissue (CALT) is crucial for ocular immune responses.
  • Understanding CALT's function is vital for ocular surface health.

Purpose of the Study:

  • To investigate the function, development, and cellular dynamics of CALT.
  • To utilize a novel mouse model for in-depth CALT analysis.

Main Methods:

  • Examined CALT expression and morphology in BALB/c mice.
  • Applied topical antigens, particles, and bacteria to study immune responses and transport.
  • Utilized two-photon microscopy for intravital visualization.

Main Results:

  • Mouse CALT development and ultrastructure resemble human CALT.
  • Topical antigen challenge significantly impacts CALT expression.
  • Demonstrated bacterial translocation via lymphoepithelium with cellular velocities around 8 µm/min.

Conclusions:

  • Mouse CALT acts as a dynamic immunological interface for the ocular surface.
  • CALT exhibits morphological plasticity, particle/bacteria transport, and cellular migration.
  • This study enhances understanding of ocular immune mechanisms.
Abstract

More Related Videos

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

13.8K
Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy08:21

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Published on: October 2, 2018

6.2K

Related Concept Videos

Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
3.4K
Secondary Lymphoid Organs01:15

Secondary Lymphoid Organs

Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
7.2K
Microbiome of the Eye01:22

Microbiome of the Eye

The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
67
Detailed Structure and Function of Lymph Nodes01:23

Detailed Structure and Function of Lymph Nodes

Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
13.4K
Introduction to Lymphatic and Immune System01:23

Introduction to Lymphatic and Immune System

Immunity is a crucial biological concept about our body's inherent capacity to prevent infections and diseases. A complex network of cells and tissues collectively known as the immune system facilitates this natural defense mechanism. The immune system plays an integral role in maintaining our health and well-being, shielding us from potential health threats.
The immune responses can be categorized into two types: innate and adaptive. Innate immunity comprises nonspecific defenses we are born...
12.1K
Functions of the Lymphatic and Immune System01:28

Functions of the Lymphatic and Immune System

The lymphatic system plays a crucial role in bolstering our immune system. It consists of a network of lymphoid organs, lymph, and lymphatic vessels that provide structural and functional support in safeguarding the body against pathogens such as viruses and bacteria.
The primary lymphoid organs, including the bone marrow and the thymus, serve as the maturation sites for lymphocytes. Secondary lymphoid organs, like the mucosa-associated lymphoid tissue, activate these lymphocytes and serve as...
6.4K