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Updated: May 1, 2026

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

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[Pathologic diagnosis of mature B-cell lymphoma]

Naoya Nakamura

    Nihon Rinsho. Japanese Journal of Clinical Medicine
    |April 15, 2014
    PubMed

    Insights

    Pathologic diagnosis of mature B-cell lymphoma (BCL) relies on identifying surface immunoglobulin (sIg) via flow cytometry (FCM). Further immunohistochemistry (IHC) is crucial for differentiating low-grade from high-grade BCL subtypes.

    Area of Science:

    • Hematopathology
    • Oncology
    • Immunology

    Context:

    • Accurate diagnosis of mature B-cell lymphomas (BCLs) is critical for appropriate patient treatment.
    • Mature B-cells are characterized by surface immunoglobulin (sIg), detectable by flow cytometry (FCM) but not immunohistochemistry (IHC).
    • BCLs are graded based on proliferation patterns and MIB-1 index, necessitating specific diagnostic markers.

    Purpose:

    • To outline the diagnostic procedure for mature B-cell lymphomas (BCLs).
    • To define mature B-cells and their detection methods.
    • To detail the markers required for differentiating BCL subtypes.

    Summary:

    • Mature B-cell lymphoma (BCL) diagnosis involves identifying surface immunoglobulin (sIg) using flow cytometry (FCM).
    • Subclassification into low-grade and high-grade BCL is based on cellular proliferation and MIB-1 index.
    • Immunohistochemistry (IHC) with markers like CD5, CD10, BCL-2, BCL-6, MUM-1, and cyclin D1 is essential for precise diagnosis.

    Impact:

    • Establishes a clear diagnostic pathway for mature B-cell lymphomas.
    • Highlights the complementary roles of FCM and IHC in BCL subtyping.
    • Aids in distinguishing between low and high-grade BCL for targeted therapy selection.

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