Related Experiment Video

Updated: Aug 9, 2026

In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
13:10

In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

Published on: August 1, 2014

[Epidermal reticular CD1 pattern]

H W Niedecken1, G Lutz, M Uerlich

  • 1Universitäts-Hautklinik und Poliklinik, Rheinische Friedrich-Wilhelms-Universität Bonn.

Zeitschrift Fur Hautkrankheiten
|September 15, 1989
PubMed

Insights

Researchers studied skin biopsies using immunoperoxidase techniques. They found a pemphigus-like pattern linked to free CD1 antigen from Langerhans cells in intercellular spaces.

Area of Science:

  • Dermatopathology
  • Immunohistochemistry
  • Cell Biology

Context:

  • The avidin-biotin complex immunoperoxidase technique was employed.
  • Monoclonal antibody anti-Leu 6, targeting the Langerhans cell antigen CD1, was utilized.
  • 183 biopsies from various skin diseases and normal skin were analyzed.

Purpose:

  • To investigate the intercellular pattern observed in skin biopsies.
  • To determine the origin and nature of the stained antigen in intercellular spaces.

Summary:

  • A reticular, pemphigus-like intercellular pattern was identified in 47 out of 183 cases.
  • This pattern showed no correlation with specific skin diseases, inflammatory infiltrates, HLA-DR expression, or Langerhans cell counts.
  • The pattern was consistently absent in aerosyringia and deep infundibula, correlating with the presence of Langerhans cells.

Impact:

  • The study suggests the stained antigen is free CD1, originating from Langerhans cells and diffusing into keratinocytic intercellular spaces.
  • This finding provides insights into Langerhans cell behavior and antigen distribution in the epidermis.
  • The research may contribute to understanding intercellular dermatopathological processes.

More Related Videos

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture08:32

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture

Published on: October 20, 2023

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Epithelial Tissues and Their Functions01:23

Epithelial Tissues and Their Functions

Epithelial tissues are large sheets of cells covering all of the surfaces of the body. These surfaces can be internal or external, for example, skin, airways, the digestive tract, the urinary system, and the reproductive system. Hollow organs and body cavities that do not connect to the body's exterior, including blood vessels and serous membranes, are lined by epithelial tissue known as the endothelium.
Epithelial tissues provide the body's first line of protection from physical, chemical, and...
Cells of the Epidermis01:24

Cells of the Epidermis

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
Papillary Dermis01:11

Papillary Dermis

Dermis
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
Layers of the Epidermis01:21

Layers of the Epidermis

The epidermis, the outermost layer of the skin, is composed of several distinct layers. From deep to superficial, the layers of the epidermis are as follows:
Stratum Basale
Stratum basale, also known as the stratum germinativum, is the deepest layer of the epidermis. It is composed of a single layer of actively dividing cells called basal cells or basal keratinocytes. These cells constantly undergo cell division to replenish the upper layers of the epidermis. Additionally, melanocytes, which...
Reticular Dermis01:15

Reticular Dermis

The papillary and reticular dermis are the two layers of the dermis. They are made of connective tissue with fibers of collagen extending from one to the other, making the border between the two somewhat indistinct. The dermal papillae extending into the epidermis belong to the papillary layer, whereas the dense collagen fiber bundles below belong to the reticular layer.
Reticular Layer
Underlying the papillary layer is the much thicker reticular layer, composed of dense, irregular connective...