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Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine
Anna Hillström1, Ragnvi Hagman, Harold Tvedten
1Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Insights
A new canine-specific C-reactive protein (CRP) assay offers reliable measurement for diagnosing and monitoring inflammatory diseases in dogs. This automated method provides accurate results, enhancing clinical veterinary diagnostics.
Area of Science:
- Veterinary Diagnostics
- Immunochemistry
- Canine Medicine
Background:
- C-reactive protein (CRP) is a key biomarker for systemic inflammation in dogs.
- Existing human CRP assays show unpredictable cross-reactivity with canine CRP.
- Automated assays are needed for efficient and accurate canine CRP measurement.
Purpose of the Study:
- To validate a novel automated canine-specific immunoturbidimetric CRP assay (Gentian cCRP).
- To assess the reliability and suitability of the new assay for clinical veterinary use.
Main Methods:
- Evaluation of imprecision, accuracy, linearity, prozone effect, and interference.
- Stability testing under various storage conditions (4°C, 22°C).
- Comparison with a human CRP assay and establishment of a reference interval in healthy dogs.
Main Results:
- The Gentian cCRP assay demonstrated high precision (<2.4% imprecision) and linearity.
- No prozone effect or interference from common substances was detected.
- CRP was stable for 14 days, and the assay showed good agreement with a validated human assay.
Conclusions:
- The canine-specific immunoturbidimetric CRP assay is a reliable and rapid diagnostic tool.
- Its automated nature makes it highly suitable for routine clinical application in veterinary medicine.
- This assay improves the accuracy of CRP measurement in canine patients.
Background:
Measurement of C-reactive protein (CRP) is used for diagnosing and monitoring systemic inflammatory disease in canine patients. An automated human immunoturbidimetric assay has been validated for measuring canine CRP, but cross-reactivity with canine CRP is unpredictable.
Objective:
The purpose of the study was to validate a new automated canine-specific immunoturbidimetric CRP method (Gentian cCRP).
Methods:
Studies of imprecision, accuracy, prozone effect, interference, limit of quantification, and stability under different storage conditions were performed. The new method was compared with a human CRP assay previously validated for canine CRP determination. Samples from 40 healthy dogs were analyzed to establish a reference interval.
Results:
Total imprecision was < 2.4% for 4 tested serum pools analyzed twice daily over 10 days. The method was linear under dilution, and no prozone effect was detected at a concentration of 1200 mg/L. Recovery after spiking serum with purified canine CRP at 2 different concentrations was 123% and 116%, respectively. No interference from hemoglobin or triglycerides (10 g/L) was detected. CRP was stable for 14 days at 4°C and 22°C. In the method comparison study, there was good agreement between the validated human CRP assay and the new canine-specific assay. Healthy dogs had CRP concentrations that were less than the limit of quantification of the Gentian cCRP method (6.8 mg/L).
Conclusions:
The new canine-specific immunoturbidimetric CRP assay is a reliable and rapid method for measuring canine CRP, suitable for clinical use due to the option for an automated assay.
