Related Experiment Video
Updated: Apr 27, 2026
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
Immunoglobulin Heavy Chain Gene Rearrangement in Non B-cell Haematological Malignancies
M N Noor Haslina1, R Marini2, B Rosnah2
1Haematology Department, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. drhaslina@kb.usm.my.
Insights
Immunoglobulin heavy chain (IGH) gene rearrangement detection via PCR is key for B-cell cancers. This study found IGH rearrangement in some T-cell acute lymphoblastic leukemia and acute myeloid leukemia cases, though less common than in B-cell malignancies.
Area of Science:
- Hematology
- Oncology
- Molecular Diagnostics
Background:
- Clonality detection using immunoglobulin heavy chain (IGH) gene rearrangements via polymerase chain reaction (PCR) is crucial for diagnosing B-lymphoid malignancies.
- IGH gene rearrangement serves as an optimal target for assessing clonality in B-lymphoid cancers.
Purpose of the Study:
- To evaluate the presence of IGH gene rearrangement in non B-cell hematologic malignancies.
- To investigate IGH gene rearrangement in T-cell acute lymphoblastic leukemia (T-ALL), acute myeloid leukemia (AML), and biphenotypic leukemia.
Main Methods:
- Studied 18 cases of hematological malignancies.
- Included five patients with T-ALL, 12 with AML, and one with biphenotypic leukemia.
- Utilized PCR to amplify IGH gene rearrangements.
Main Results:
- IGH gene rearrangement was detected in 60% of T-ALL cases (3 out of 5).
- IGH gene rearrangement was found in 16.7% of AML cases (2 out of 12).
- The single biphenotypic leukemia case tested negative for IGH gene rearrangement.
Conclusions:
- While IGH gene rearrangement is characteristic of B-cell malignancies, it can occur in a small subset of T-cell and myeloid malignancies.
- This finding highlights the potential for IGH gene rearrangement as a diagnostic marker beyond B-cell lineage.
Objective:
Clonality detection through amplifying immunoglobulin heavy chain (IGH) gene rearrangements by polymerase chain reaction (PCR) is a useful tool in diagnosis of various B-lymphoid malignancies. Immunoglobulin heavy chain gene rearrangement can be an optimal target for clonality detection in B-lymphoid malignancies. In the present study, we evaluated the presence of IGH gene rearrangement in non B-cell haemato-oncology patients including T-cell acute lymphoblastic leukaemia (T-ALL), acute myeloblastic leukaemia (AML) and biphenotypic leukaemia.
Mehtods:
We studied 18 cases of haematological malignancies which comprised five patients with T-ALL, 12 patients with AML and one with biphenotypic leukaemia.
Results:
We found that the incidence of IGH gene rearrangement in T-ALL and AML were three (60%) and two (16.7%), respectively. The patient with biphenotypic leukaemia was negative for IGH gene rearrangement.
Conclusion:
Immunoglobulin gene rearrangement, which occurs in almost all haematological malignancies of B-cell lineage, also presents in a very small proportion of T-cell or myeloid malignancies.
More Related Videos
15:07VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
Published on: December 28, 2015
09:35Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
Published on: April 20, 2021
