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Serial transfer of single-cell-derived immunocompetence reveals stemness of CD8(+) central memory T cells

Patricia Graef1, Veit R Buchholz1, Christian Stemberger2

  • 1Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich 81675, Germany.

Immunity
|July 19, 2014
PubMed

Insights

Adult tissue stem cells reside within CD62L(+) central memory T (Tcm) cells. These stem cells possess self-renewal capacity and multipotency, crucial for reconstituting immunocompetence and offering therapeutic potential.

Area of Science:

  • Immunology
  • Cell Biology
  • Stem Cell Research

Background:

  • Immunological memory is vital for adaptive immunity.
  • The role of stem-cell-like lymphocytes in maintaining memory is hypothesized but not fully established.
  • Previous data focused on developmental potential, lacking functional hallmarks of stemness.

Purpose of the Study:

  • To investigate the self-renewal capacity and multipotency of individual memory lymphocytes.
  • To identify the specific lymphocyte subset acting as adult tissue stem cells.
  • To explore the therapeutic potential of identified stem cell populations.

Main Methods:

  • In vivo fate mapping of CD8(+) T cells.
  • Serial single-cell adoptive transfer across three generations.
  • Infection-driven re-expansion using Listeria monocytogenes.

Main Results:

  • Immune responses from single naive T (Tn) cells, primary, and secondary central memory T (Tcm) cells showed similar size, diversity, and stochastic variation.
  • These responses successfully reconstituted immunocompetence against a lethal bacterial infection.
  • The CD62L(+) Tcm cell compartment was identified as the residence of adult tissue stem cells.

Conclusions:

  • Adult tissue stem cells are present within the CD62L(+) Tcm cell population.
  • These stem cells exhibit self-renewal and multipotency, essential for long-term immune defense.
  • The CD62L(+) Tcm cell subset holds significant promise for therapeutic applications in immunology.

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