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Published on: November 16, 2015
[Immunohistological study on immunocompetent cells in palatine tonsil and pharyngeal tonsil--the quantitative study
Insights
Immunocompetent cell distribution in palatine tonsils and pharyngeal tonsils was analyzed. Specific immune cell changes were observed in recurrent tonsillitis, otitis media, and nasal allergy cases.
Area of Science:
- Immunology
- Otorhinolaryngology
- Cell Biology
Background:
- Palatine tonsils and pharyngeal tonsils are key components of the upper respiratory airway's local immunity.
- Understanding the distribution of immunocompetent cells within these tissues is crucial for diagnosing and managing related conditions.
Purpose of the Study:
- To quantitatively investigate the distribution of various immunocompetent cells (IgG, IgM, IgA, IgD, IgE, CD4, CD8) in tonsils and adenoids.
- To correlate these distributions with conditions such as recurrent tonsillitis, otitis media with effusion, and nasal allergy.
Main Methods:
- Immunohistological analysis using monoclonal antibodies to identify specific cell types.
- Quantitative assessment of cell proportions using an image analyzer (CUE-2, Olympus, USA).
Main Results:
- No significant difference in overall Ig-positive cells and T cell subsets between tonsils and adenoids.
- Increased IgG- and IgD-positive cells in tonsils with recurrent tonsillitis.
- Altered IgA2-positive cell distribution in tonsils and adenoids associated with otitis media with effusion.
- Elevated IgE-positive cells in both tonsils and adenoids in cases of nasal allergy.
Conclusions:
- The image analyzer provides a breakthrough for quantitative immunohistological studies of tonsils and adenoids.
- Specific immune cell patterns in tonsils and adenoids offer insights into local immunity and disease pathogenesis in the upper airway.
Abstract:
Distribution of various immunocompetent cells, i.e., IgG-, IgM-, IgA-, IgD-, IgE-, CD4-, and CD8-positive cells in palatine tonsils (tonsils) and pharyngeal tonsils (adenoids) of same patients were investigated immunohistologically by using monoclonal antibodies. The proportion of Ig-positive cells and T cell subsets in both tonsils were quantitatively studied by the image analyzer (CUE-2, Olympus, USA). The results were summarized as follows: 1) There was no significant difference in proportions of Ig-positive cells and T cell subsets between tonsils and adenoids. 2) IgG- and IgD-positive cells in the tonsils were significantly increased in cases with recurrent tonsillitis than in those with simple tonsillar hypertrophy. 3) Number of IgA2-positive cells were significantly changed, i.e., decreased in the subepithelial area of tonsils and increased in the interfollicular area of adenoids in cases complicated with otitis media with effusion. 4) In cases complicated with nasal allergy, IgE-positive cells were significantly increased in the interfollicular area in both of those tonsils and adenoids. These results indicate that the image analyzer will be a breakthrough in quantitative study on the immunohistology of tonsils and adenoids and give us useful informations about roles of the tonsil and the adenoid on the local immunity of the upper respiratory airway.
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