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Specimen recentrifugation and elevated troponin I levels

Simone Canovi1, Daniele Campioli2, Luigi Marcheselli3

  • 1Department of Laboratory Medicine, Clinical Analysis Laboratory, University Hospital of Modena, and simone.canovi@studio.unibo.it.

Laboratory Medicine
|January 25, 2015
PubMed

Insights

Recentrifugation of plasma specimens significantly reduces cardiac troponin I levels, potentially leading to patient misclassification. This practice also unnecessarily prolongs laboratory turnaround times.

Area of Science:

  • Clinical Chemistry
  • Biomarker Analysis
  • Laboratory Medicine

Background:

  • Cardiac troponin I (cTnI) is a critical biomarker for diagnosing myocardial infarction.
  • Accurate cTnI measurements are essential for timely and correct patient management.
  • Specimen handling procedures can impact biomarker concentrations.

Purpose of the Study:

  • To investigate the effect of recentrifugation on plasma troponin I levels.
  • To determine if recentrifugation alters cTnI concentrations in clinically relevant samples.

Main Methods:

  • 189 plasma specimens with elevated troponin I (>60 ng/L) were recentrifuged and re-assayed.
  • Statistical analysis (Wilcoxon test, Passing-Bablok regression) compared pre- and post-recentrifugation values.

Main Results:

  • A statistically significant decrease in troponin I was observed after recentrifugation (P <.01).
  • Specimens near the cutoff showed a median reduction of 10 ng/L.
  • Confidence intervals indicated a consistent decrease in cTnI concentrations.

Conclusions:

  • Recentrifugation of plasma reduces elevated troponin I levels.
  • This reduction poses a risk of misclassifying patients with cardiac events.
  • The practice also leads to extended laboratory turnaround times.
Abstract

Keywords:
cardiac biomarkerscardiovascular diseaseclinical chemistrypreanalysisrecentrifugationtroponin I

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