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CD244 is expressed on dendritic cells and regulates their functions

Anna-Maria Georgoudaki1, Sorosh Khodabandeh1, Speranta Puiac2

  • 1Department of Medicine, Center for Infectious Medicine, F59, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.

Immunology and Cell Biology
|February 4, 2015
PubMed

Insights

Signaling lymphocytic activation molecule (SLAM) receptor CD244 is present on dendritic cells (DCs). Its absence in mice boosts pro-inflammatory cytokine production and enhances natural killer (NK) cell activation.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Signaling lymphocytic activation molecule (SLAM) receptors are crucial for immune responses, influencing NK cell cytotoxicity and cytokine production.
  • The SLAM receptor CD244 (2B4, SLAMf4) is expressed on various immune cells, with its functions primarily studied in NK and T cells.

Purpose of the Study:

  • To investigate the expression and function of CD244 in murine dendritic cell (DC) subsets.
  • To determine the role of CD244 in DC maturation, cytokine production, and interaction with NK cells.

Main Methods:

  • Analysis of CD244 expression across different murine DC subsets.
  • Comparison of CD86 and MHC class II expression on DCs from CD244(-/-) and wild-type mice.
  • Assessment of cytokine production by DCs upon Toll-like receptor (TLR) stimulation.
  • Evaluation of NK cell activation by DCs in vitro.

Main Results:

  • All examined murine DC subsets express CD244, with varying expression levels.
  • DCs from CD244(-/-) mice showed reduced CD86 and MHC class II expression.
  • Absence of CD244 led to significantly higher pro-inflammatory cytokine production by splenic DCs upon TLR stimulation (LPS and CpG).
  • DCs from CD244(-/-) mice induced increased NK cell activation in vitro.

Conclusions:

  • CD244 is expressed on all murine DC subsets and modulates their function.
  • CD244 deficiency in DCs results in enhanced pro-inflammatory responses and increased NK cell activation.
  • CD244 is identified as an immuno-modulatory receptor on dendritic cells.

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