Related Experiment Video

Updated: Aug 8, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Circulating human T and B lymphocytes express the p55 interleukin-2 receptor molecule (TAC, CD25)

H Zola1, B X Mantzioris, J Webster

  • 1Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, Australia.

Immunology and Cell Biology
|August 1, 1989
PubMed

Insights

A novel immunofluorescence method detects CD25-positive lymphocytes in healthy adults. This technique reveals a significant population of these cells, previously undetectable by standard methods, across various lymphocyte subsets.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The CD25 antigen (interleukin-2 receptor alpha chain) is a marker expressed on activated lymphocytes.
  • Standard immunofluorescence techniques have limitations in detecting low-abundance cell populations.

Purpose of the Study:

  • To develop and validate a highly sensitive immunofluorescence assay for detecting CD25-positive lymphocytes in normal human blood.
  • To characterize the lymphocyte subsets expressing CD25 in healthy individuals.

Main Methods:

  • Utilized a highly sensitive immunofluorescence procedure.
  • Tested peripheral blood lymphocytes from ten healthy adult donors.
  • Employed three distinct anti-CD25 monoclonal antibodies.
  • Conducted double-marker studies to identify positive cell types.

Main Results:

  • 15-45% of blood lymphocytes from normal donors reacted with anti-CD25 antibodies using the sensitive method.
  • All ten tested donors consistently showed a population of CD25-positive cells.
  • Positive cells were identified as B cells, CD4+ T cells, and CD8+ T cells.

Conclusions:

  • A sensitive immunofluorescence assay can detect a significant population of CD25-expressing lymphocytes in healthy individuals.
  • These CD25-positive cells represent diverse lymphocyte subsets, including B and T cells.
  • The findings highlight the prevalence of CD25 expression in normal immune systems, challenging previous detection limits.

More Related Videos

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice07:07

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Published on: June 27, 2020

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD22608:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

Related Concept Videos

Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...