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Published on: January 16, 2013
Determination of CD64 for the Diagnosis of Bacterial Chronic Prostatitis
Li Qian1, Shi-Bao Li2, Yan Zhou1
1Department of Medicine Laboratory, The Second People's Hospital of Lianyungang, Xinpu, China.
Insights
Leukocyte CD64 expression in prostatic fluid can help diagnose chronic prostatitis (CP). Flow cytometry detected higher CD64 levels in infected patients, offering a rapid diagnostic marker for bacterial prostatitis.
Area of Science:
- Immunology
- Urology
- Medical Diagnostics
Background:
- CD64 is a high-affinity immunoglobulin (IgG) receptor expressed on leukocytes.
- Chronic prostatitis (CP) diagnosis can be challenging.
- Leukocyte CD64 expression may indicate inflammatory processes.
Purpose of the Study:
- To investigate the clinical significance of leukocyte CD64 expression in prostatic fluid for diagnosing chronic prostatitis (CP).
- To evaluate flow cytometry as a diagnostic tool for CP.
Main Methods:
- Prostatic fluid samples were collected from 116 CP patients and 27 controls.
- Bacterial cultures were performed.
- Leukocyte CD64 expression was determined using flow cytometry and monoclonal antibodies.
Main Results:
- Leukocyte CD64 expression levels were significantly higher in CP patients with bacterial infections compared to controls.
- Elevated CD64 expression was noted for Staphylococcus simulans and Staphylococcus auricularis infections.
- Flow cytometry provided faster and more accurate detection of leukocyte CD64 expression than bacterial culture.
Conclusions:
- Leukocyte CD64 expression in prostatic fluid correlates with bacterial infection in chronic prostatitis.
- CD64 may serve as a valuable biomarker for the early diagnosis of CP.
- Flow cytometry offers a promising method for rapid CP diagnosis.
Problem:
CD64 is a 72-kDa transmembrane glycoprotein, the highest affinity receptor of immunoglobulin (IgG), and a member of the IgG superfamily. The CD64 expression by leukocytes in the prostatic fluid was determined by flow cytometry to explore the clinical significance of this glycoprotein in the diagnosis of chronic prostatitis (CP).
Method Of Study:
Prostatic fluid samples collected from 116 CP patients and 27 normal cases were subjected to bacterial culture and leukocyte CD64 expression determination using flow cytometry with monoclonal antibodies.
Results:
The leukocyte CD64 expression levels of the pathogen groups were significantly higher than those of the normal groups, especially those of Staphylococcus simulans (3879.26 ± 764.52/cell) and Staphylococcus auricularis (2903.14 ± 137.61/cell). The leukocyte CD64 expression in the prostatic fluid was more rapidly and accurately detected by flow cytometry than by bacterial culture.
Conclusions:
CD64 expression by leukocytes in the prostatic fluid correlated with bacterial infection and may serve as a marker for the early diagnosis of CP.
