Related Experiment Video
Updated: Apr 10, 2026
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
Published on: March 8, 2013
Hybrid lymph node imaging using 64Cu-labeled mannose-conjugated human serum albumin with and without indocyanine
Choong Mo Kang1, Gwang Il An, Yearn Seong Choe
1aDepartment of Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine bDepartment of Health Sciences and Technology, SAIHST, Sungkyunkwan University cMolecular Imaging Research Center, Korea Institute of Radiological & Medical Sciences, Seoul, Korea.
Insights
This study developed novel hybrid imaging probes using 64Cu-labeled mannose-conjugated human serum albumin. The probes demonstrated selective uptake in lymph nodes, with one variant showing enhanced specificity for mannose receptors.
Area of Science:
- Biomedical Imaging
- Radiochemistry
- Nanotechnology
Background:
- Human serum albumin (HSA) serves as a versatile platform for developing hybrid imaging probes due to its abundant lysine residues, single cysteine residue, and indocyanine green (ICG) adsorption capabilities.
- Developing multifunctional imaging probes is crucial for advanced diagnostic applications.
Purpose of the Study:
- To synthesize and evaluate 64Cu-labeled mannose-conjugated HSA probes, with and without ICG ([64Cu]1-ICG and [64Cu]1, respectively).
- To compare hybrid positron emission tomography/near-infrared fluorescence (PET/NIRF) imaging with PET/Cerenkov luminescence (CL) imaging for lymph node (LN) visualization.
Main Methods:
- Synthesis of 1,4,7,10-Tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA)/mannose-conjugated HSA (1) via conjugation of mannose to lysine residues and DOTA to a cysteine residue.
- Radiolabeling of compound 1 with 64Cu to form [64Cu]1, followed by ICG adsorption to create [64Cu]1-ICG.
- In vivo imaging studies in mice using PET/NIRF and PET/CL modalities after injection into foot pads, complemented by biodistribution analysis.
Main Results:
- Successful site-specific conjugation of mannose (7.17 ± 0.49) and DOTA (0.95 ± 0.18) molecules to HSA.
- High efficiency (96.0 ± 1.1%) achieved for 64Cu-labeling using a single DOTA molecule.
- Probes showed avid uptake in popliteal lymph nodes (PO), with [64Cu]1 exhibiting a slightly higher popliteal to lumbar lymph node uptake ratio compared to [64Cu]1-ICG.
Conclusions:
- In vivo studies indicate that [64Cu]1 demonstrates more specific and selective binding to mannose receptors in the popliteal lymph nodes than [64Cu]1-ICG.
- The findings support the potential of [64Cu]1 as a targeted imaging agent for lymph nodes.
Objective:
Human serum albumin (HSA), which has 58 Lys residues, one Cys residue, and indocyanine green (ICG) adsorption sites, can be used as a multifunctional platform for the development of hybrid imaging probes. In this study, we prepared 64Cu-labeled mannose-conjugated HSA with and without ICG ([64Cu]1-ICG and [64Cu]1, respectively) and compared hybrid PET/near-infrared fluorescence (NIRF) imaging with positron emission tomography (PET)/Cerenkov luminescence (CL) imaging of lymph nodes (LNs).
Materials And Methods:
1,4,7,10-Tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA)/mannose-conjugated HSA (1) was synthesized by conjugating mannose molecules to Lys residues and a DOTA molecule to a Cys residue of HSA. Compound 1 was then labeled with Cu ([64Cu]1), and the resulting [64Cu]1 was adsorbed with ICG ([64Cu]1-ICG). PET/NIRF or PET/CL imaging and subsequent biodistribution studies were performed in ICR mice after injection of the probes into the foot pads.
Results:
The numbers of mannose and DOTA molecules conjugated to HSA were 7.17 ± 0.49 and 0.95 ± 0.18, respectively. The site-specific conjugation of one DOTA molecule to HSA was sufficient for 64Cu-labeling with high efficiency (96.0 ± 1.1%). PET/NIRF and PET/CL imaging and subsequent biodistribution studies demonstrated that the probes were avidly taken up by the popliteal LNs (PO), with a slightly higher uptake ratio of the PO to the lumbar LNs by [64Cu]1.
Conclusion:
In-vivo studies suggest that [64Cu]1 has more specific and selective binding to mannose receptors in the PO than [64Cu]1-ICG.
More Related Videos
06:37Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
Published on: October 20, 2010
11:34Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
Published on: April 29, 2017