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[Vimentin-positive epithelial cells in aggregated lymphoid nodules (Peyer's patches) in rabbits]
Insights
Specific M-cells in rabbit ileum aggregated lymphoid nodules (ALN) possess unique vimentin cytoskeleton. These M-cells interact with interepitheliocytic lymphocytes, suggesting a role in immune surveillance within the gut.
Area of Science:
- Cell Biology
- Immunology
- Gastroenterology
Context:
- The ileum's aggregated lymphoid nodules (ALN) are crucial for gut immunity.
- Understanding the cellular composition and function of the ALN epithelium is vital.
Purpose:
- To investigate the cytoskeleton peculiarities of membranous cells in the ALN cupola epithelium.
- To identify and characterize vimentin-positive cells within the ALN.
Summary:
- Vimentin-positive epitheliocytes, identified as M-cells, were found in the lateral part of the rabbit ileum's ALN cupola epithelium.
- These M-cells exhibit distinct interactions with interepitheliocytic lymphocytes, forming contacts near the base and surrounding them near the apex.
- These cells were absent in intestinal crypts, villi, and cupola apices.
Impact:
- This study identifies M-cells based on vimentin expression in the ALN.
- Provides insights into the structural organization and potential immune functions of M-cells within the gut-associated lymphoid tissue.
Abstract:
Peculiarities of cytoskeleton in membranous cells and disposition of the latter in the cupola epithelium in aggregated lymphoid nodules++ (ALN) have been studied in the ileum of 5 rabbits. The material has been fixed in liquid nitrogen and in the mixture of paraformaldehyde and glutar aldehyde. Methods of immunomorphology, high resolving light and transmissive electron microscopy have been used. Monoclonal antibodies to vimentin are selectively bind with a specific population of the ALN cupola epithelial cells. These cells are regularly arranged in the epithelium of the cupola lateral part and they are absent in the epithelium of the intestinal crypts, villi and apex of the cupolas. In the lateral epithelium of the cupolas surface, nearer to their base vimentin-positive++ epitheliocytes make contacts with single interepitheliocytic lymphocytes, and nearer to the apex they surround compact groups of the interepitheliocytic lymphocytes. The vimentin-positive++ epitheliocytic cells are identified as M-cells.