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Flow cytometry in hematological nonmalignant disorders
M C Béné1, Y Le Bris1, N Robillard1
1Hematology Biology, University Hospital of Nantes, Nantes, France.
Insights
Multiparameter flow cytometry (MFC) aids in diagnosing blood cancers. This review explores MFC
Area of Science:
- Hematology
- Immunophenotyping
- Clinical Laboratory Science
Background:
- Multiparameter flow cytometry (MFC) is crucial for diagnosing hematological malignancies.
- Its utility extends to nonmalignant and premalignant hematologic disorders.
- Understanding normal leukocyte subset immunophenotypes is essential.
Purpose of the Study:
- To review the applications of MFC beyond malignancy diagnosis.
- To describe normal leukocyte immunophenotypic characteristics and their alterations.
- To highlight MFC's role in specific hematologic conditions.
Main Methods:
- Review of existing literature on MFC applications in hematology.
- Description of normal peripheral blood leukocyte subset immunophenotypes.
- Discussion of MFC marker utility in various clinical scenarios.
Main Results:
- MFC provides insights into normal leukocyte subset profiles.
- Specific markers are valuable for evaluating hyperlymphocytosis, infections, hypereosinophilia, paroxysmal nocturnal hemoglobinuria, and large granular lymphocyte disorders.
- MFC applications are expanding to include red blood cell and platelet analyses.
Conclusions:
- Multiparameter flow cytometry is a versatile tool in hematology laboratories.
- MFC aids in the characterization of various nonmalignant and premalignant hematologic conditions.
- Further developments promise broader applications of MFC in blood cell analysis.
Abstract:
Multiparameter flow cytometry (MFC) has become an integral part of the diagnosis and classification of hematological malignancies. However, several nonmalignant or premalignant disorders may benefit from this technology in hematology laboratories. This review provides information on the normal immunophenotypic characteristics of peripheral blood leukocyte subsets and their modifications in several clinical conditions. The usefulness of MFC and the specific markers that can be investigated in hyperlymphocytosis, infection, hypereosinophilia, paroxysmal nocturnal hemoglobinuria, and large granular lymphocyte disorders is described. Mention is also made of the developments of MFC for analyses of red blood cells or platelets.
