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Updated: Mar 30, 2026

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Elispot Igra With Purified Protein Derivative Stimulation For Diagnosing Nontuberculous Mycobacterial Cervical

Antti Kontturi1, Tamara Tuuminen, Riitta Karttunen

  • 1From the *Children's Hospital, University of Helsinki and Helsinki University Central Hospital, Helsinki; †Eastern Finland Laboratory Centre Joint Authority Enterprise (ISLAB), Mikkeli District Laboratory, Mikkeli; ‡Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki; and §Laboratory Division (HUSLAB), Helsinki University Central Hospital, Helsinki, Finland.

The Pediatric Infectious Disease Journal
|November 20, 2015
PubMed

Insights

Diagnosing nontuberculous mycobacterial lymphadenitis in children is challenging. A modified T-SPOT.TB test using purified protein derivative shows promise as a noninvasive diagnostic method with high accuracy.

Area of Science:

  • Pediatrics
  • Infectious Diseases
  • Immunology

Background:

  • Childhood cervical lymphadenitis is frequently caused by nontuberculous mycobacteria.
  • Accurate diagnosis of this condition presents a significant clinical challenge.

Purpose of the Study:

  • To introduce and evaluate a novel, noninvasive diagnostic method for childhood nontuberculous mycobacterial lymphadenitis.

Main Methods:

  • A modified T-SPOT.TB test was developed.
  • Purified protein derivative (PPD) was incorporated as an additional antigen in the test.

Main Results:

  • The modified T-SPOT.TB test demonstrated high diagnostic performance.
  • Estimated sensitivity was 1.00, and estimated specificity was 0.81.

Conclusions:

  • The modified T-SPOT.TB test is a promising noninvasive tool for diagnosing childhood nontuberculous mycobacterial lymphadenitis.
  • This method offers improved diagnostic capabilities for clinicians facing this challenging condition.

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