Polydimethylsiloxane microfluidic chemiluminescence immunodevice with the signal amplification strategy for sensitive
Huifang Li1, Mei Zhao1, Wei Liu1
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
Insights
This study presents a novel microfluidic immunodevice for highly sensitive human immunoglobulin G (IgG) detection. The device utilizes signal amplification strategies for improved clinical diagnostic applications.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunosensing
Background:
- Sensitive detection of human immunoglobulin G (IgG) is crucial for clinical diagnostics and therapeutic monitoring.
- Existing methods may lack the required sensitivity or efficiency for certain applications.
- Microfluidic devices offer advantages in sample handling and assay miniaturization.
Purpose of the Study:
- To develop a polydimethylsiloxane (PDMS) microfluidic chemiluminescence (CL) immunodevice for sensitive human IgG detection.
- To enhance detection sensitivity through signal amplification strategies.
- To evaluate the device's performance for potential clinical diagnostic applications.
Main Methods:
- Covalent immobilization of capture antibodies (Abs) onto a silanized microfluidic chip.
- Utilizing gold nanoparticles (AuNPs) functionalized with horseradish peroxidase (HRP) as labels for signal amplification.
- Employing a sandwich immunoassay format with a luminol-H2O2 CL system and a double spiral flow-channel design.
Main Results:
- The developed immunodevice achieved a low detection limit (DL) of 0.03 ng/mL for human IgG.
- Demonstrated a 7.4-fold increase in detection sensitivity compared to commercial antibody-HRP conjugates.
- The multi-HRP conjugated AuNPs and spiral flow-channel design significantly contributed to high sensitivity.
Conclusions:
- The PDMS microfluidic chemiluminescence immunodevice provides a highly sensitive platform for human IgG detection.
- The signal amplification strategy effectively enhances assay sensitivity.
- This technology offers a promising alternative for sensitive human IgG detection in clinical diagnostics and therapeutics.
Abstract:
A polydimethylsiloxane (PDMS) microfluidic chemiluminescence (CL) immunodevice for sensitive detection of human immunoglobin G (IgG) with the signal amplification strategy was developed in this work. The immunodevice was prepared by covalently immobilizing capture antibodies (Abs) on the silanized microchannel of microfluidic chip. Gold nanoparticles (AuNPs) functionalized with a high molar ratio of horseradish peroxidase (HRP) were used as an Ab label for signal amplification. Using a sandwich immunoassay, the multi-HRP conjugated AuNPs can catalyze the luminol-H2O2 CL system to achieve the high sensitivity. In addition, the double spiral flow-channel was adopted here, which can still contribute to the high sensitivity. Based on signal amplification strategy, the performance of human IgG tests revealed a lower detection limit (DL) of 0.03ng/mL and showed an increase of 7.4-fold in detection sensitivity compared to a commercial Ab-HRP conjugation. This microfluidic immunodevice can provide an alternative approach for sensitive detection of human IgG in the field of clinic diagnostic and therapeutic.
