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Immunomodulatory Effects of Cinobufagin on Murine Lymphocytes and Macrophages
Yang Yu1, Hui Wang1, Xianhua Meng1
1Liaoning Medical University, Jinzhou 121001, China.
Insights
Cinobufagin (CBG), derived from ChanSu, significantly enhances immune cell activity in mice. It boosts lymphocyte proliferation, improves the CD4+/CD8+ ratio, and increases macrophage phagocytosis.
Area of Science:
- Immunology
- Pharmacology
- Traditional Chinese Medicine
Background:
- Cinobufagin (CBG) is a key bioactive compound found in ChanSu, a traditional Chinese medicine.
- CBG has demonstrated potent pharmacological activities.
- The immunomodulatory effects of CBG require further elucidation.
Purpose of the Study:
- To investigate the impact of Cinobufagin (CBG) on the functional activity of murine immune cells.
- To assess CBG's effects on macrophage phagocytosis and lymphocyte proliferation, cytokine secretion, and cell cycle.
- To determine CBG's influence on lymphocyte subsets and cell surface markers.
Main Methods:
- Preparation and culture of murine peritoneal macrophages and splenocytes.
- Assessment of macrophage phagocytic activity using MTT assays.
- Enzyme-linked immunosorbent assay (ELISA) for detecting interleukin-2 (IL-2) and IL-10 secretion.
- Flow cytometry for analyzing cell cycle distribution and cell surface markers.
Main Results:
- Cinobufagin (CBG) significantly promoted lymphocyte proliferation, an effect attenuated by Concanavalin A (ConA) or lipopolysaccharide (LPS).
- CBG treatment led to a notable increase in the CD4+/CD8+ ratio in spleen lymphocytes and enhanced the percentage of lymphocytes in the S phase.
- CBG augmented the secretion of IL-2 and IL-10 and improved the phagocytic capacity of macrophages.
Conclusions:
- Cinobufagin (CBG) exhibits significant immunomodulatory properties.
- CBG enhances the overall activity of immune cells, including lymphocytes and macrophages.
- These findings support the potential of CBG as an immune-enhancing agent.
Abstract:
Cinobufagin (CBG), a major bioactive component of the traditional Chinese medicine ChanSu, has been reported to have potent pharmacological activity. In this study, we aimed to elucidate the effects of CBG on the activity of immune cells in mice. Peritoneal macrophages and splenocytes from mice were prepared and cultured in RPMI1640 supplemented with 10% fetal bovine serum. Concanavalin (ConA), lipopolysaccharide (LPS), and CBG (0.0125, 0.05, 0.15, or 0.25 μg/mL) were added to the culture medium, and the phagocytic activity of macrophages was detected by MTT assays. Additionally, lymphocyte secretion of interleukin- (IL-)2 and IL-10 was detected by enzyme-linked immunosorbent assay, and the cell cycle distribution and cell surface markers were detected by flow cytometry. Our results demonstrated that CBG promoted lymphocyte proliferation; this effect was suppressed by combined treatment with ConA or LPS. Moreover, CBG also significantly improved the CD4(+)/CD8(+) ratio in spleen lymphocytes and increased the percentage of spleen lymphocytes in the S phase. Finally, we found that CBG enhanced the secretion of IL-2 and IL-10 and increased the phagocytosis ability of macrophages. In summary, CBG could enhance activity of immune cells.
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