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Identification of a novel protein (G210) specific to the Golgi apparatus

R Hogue-Angeletti1, R Y Xu, J O Gonatas

  • 1Department of Pathology & Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6082.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|August 1, 1989
PubMed

Insights

Researchers identified a new Golgi-specific protein in bovine tissues using monoclonal antibody 3C9. This intrinsic membrane protein

Area of Science:

  • Cell Biology
  • Biochemistry
  • Immunology

Background:

  • The Golgi apparatus is crucial for protein modification and transport.
  • Understanding the cell-specific distribution of Golgi proteins is important for cell biology.
  • Novel proteins within the Golgi apparatus are continuously being discovered.

Purpose of the Study:

  • To identify and characterize a novel protein localized to the Golgi apparatus in bovine tissues.
  • To determine the cellular distribution and membrane properties of this newly discovered protein.

Main Methods:

  • Immunohistochemistry (light and electron microscopy) using monoclonal antibody 3C9.
  • Immunoblotting to determine molecular weight.
  • Sodium carbonate extraction and Triton X-114 partitioning to assess membrane association.

Main Results:

  • Monoclonal antibody 3C9 detected a novel antigen in exocrine pancreas, gut, and thymus epithelium.
  • Immunoelectron microscopy confirmed exclusive localization to the Golgi apparatus.
  • The antigen has a molecular weight of 210,000 daltons and is an intrinsic membrane protein.

Conclusions:

  • A novel, cell-specific, intrinsic membrane protein resident in the Golgi apparatus has been identified in bovine tissues.
  • These findings support the hypothesis that Golgi apparatus composition is cell-specific.

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