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Published on: November 10, 2023
Expression and effect of serum interleukin-24 level on bone marrow mononuclear cells in children with acute leukemia
1Department of Pediatrics, Shenzhen People Hospital, Jinan University Second Clinical Medical College, Shenzhen, Guangdong Province, China.
Insights
Interleukin-24 (IL-24) levels were higher in children with acute leukemia (AL). IL-24 induced apoptosis in bone marrow mononuclear cells (BMMNCs) from AL patients in vitro, suggesting a potential therapeutic role.
Area of Science:
- Oncology
- Immunology
- Cell Biology
Background:
- Acute leukemia (AL) is a significant pediatric cancer.
- Interleukin-24 (IL-24) is a cytokine with potential roles in cancer biology.
- Understanding IL-24's role in pediatric AL is crucial for therapeutic development.
Purpose of the Study:
- To investigate IL-24 serum levels in children with acute lymphocytic leukemia (ALL) and acute myeloid leukemia (AML).
- To examine the in vitro effect of IL-24 on the apoptosis of bone marrow mononuclear cells (BMMNCs) from AL patients.
Main Methods:
- Serum IL-24 levels were measured using ELISA in 80 children (20 each in ALL, AML, non-leukemia, healthy groups).
- BMMNCs were isolated from bone marrow samples.
- Apoptosis of BMMNCs was assessed using flow cytometry after in vitro exposure to IL-24.
Main Results:
- Serum IL-24 levels were significantly elevated in children with ALL and AML compared to non-leukemia and healthy controls.
- In vitro, IL-24 treatment (50 ng/mL) significantly increased the apoptotic rate of BMMNCs from AL patients.
- No significant difference in IL-24 levels was observed between ALL and AML groups, or between non-leukemia and healthy groups.
Conclusions:
- Elevated serum IL-24 is associated with acute leukemia in children.
- IL-24 demonstrates a pro-apoptotic effect on BMMNCs from AL patients in vitro.
- IL-24 may represent a potential therapeutic target for inducing apoptosis in pediatric acute leukemia.
Abstract:
To investigate the expression of interleukin-24 (IL-24) in the children with acute leukemia (AL) and its effect on the apoptosis of bone marrow mononuclear cells (BMMNCs) in vitro. Four groups were assessed: acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), non-leukemia, and healthy groups, 20 children in each group. ELISA was used to measure IL-24 serum level. The bone marrow was taken from patients and controls. BMMNCs were isolated and the DNA was analyzed by glucose electrophoresis. Flow cytometry was used to determine BMMNC apoptosis. The serum level of IL-24 in the ALL and AML groups were significantly higher than in the other two groups. There was no statistical difference between ALL and AML groups, either between non-leukemia and healthy groups. BMMNCs were exposed to IL-24 for 48 h, and the apoptotic rate of the group treated with 50 ng/ml IL-24 was obviously higher than that of control group (0 ng/mL). The serum IL-24 level of AL children decreased comparing to non-leukemia and healthy children, indicating that IL-24 can induce BMMNCs apoptosis of AL children in vitro.
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