Related Experiment Video

Updated: Aug 8, 2026

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
09:11

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Diagnostic accuracy of the immunocytochemical study of body fluids

C Y Li1, S C Ziesmer, Y C Wong

  • 1Division of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.

Acta Cytologica
|September 1, 1989
PubMed

Insights

Immunocytochemical stains accurately characterize cells in body fluids like pleural and CSF. This method enhances cytologic diagnosis, especially for challenging cases.

Area of Science:

  • Cytopathology
  • Immunohistochemistry
  • Fluid analysis

Background:

  • Cytologic examination of body fluids is crucial for diagnosis.
  • Morphologic assessment can be challenging in certain cases.
  • Immunocytochemistry offers potential for improved diagnostic accuracy.

Purpose of the Study:

  • To evaluate the diagnostic accuracy of immunocytochemical characterization of body fluids.
  • To compare immunocytochemistry with routine morphologic examination.
  • To assess the utility of specific immunocytochemical markers.

Main Methods:

  • Evaluated 100 body fluid specimens (pleural, peritoneal, pericardial, CSF).
  • Utilized immunocytochemical markers: common-leukocyte antigen, epithelial membrane antigen, keratin, desmin.
  • Performed additional studies on CSF for neurofilaments, GFAP, vimentin, and melanoma-associated antigen.

Main Results:

  • Immunocytochemical characterization demonstrated high accuracy.
  • Confirmed the reliability of using a panel of immunocytochemical stains.
  • Specific markers were applied based on fluid type and diagnostic need.

Conclusions:

  • Immunocytochemistry is an accurate method for body fluid cell characterization.
  • Recommended as an adjunct to improve cytologic diagnosis accuracy.
  • Particularly beneficial for diagnostically difficult morphologic features.

More Related Videos

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum10:19

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Published on: November 23, 2013

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry10:58

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...