Related Experiment Video

Updated: Mar 26, 2026

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
15:07

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

Published on: December 28, 2015

27.4K

Clonal relationships in recurrent B-cell lymphomas

Seung Eun Lee1, So Young Kang2, Hae Yong Yoo3

  • 1Department of Pathology, Konkuk University School of Medicine, Konkuk University Medical Center, Seoul, Korea.

Oncotarget
|February 6, 2016
PubMed

Insights

Relapse in B-cell lymphomas can originate from an unrelated clone, though uncommon. Sequencing immunoglobulin (Ig) genes, not just PCR fragment size, is crucial for determining clonal relationships between primary and relapsed tumors.

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Immunoglobulin (Ig) gene rearrangements are typically stable during neoplastic cell clonal expansion.
  • Understanding clonal relationships in lymphoma relapse is critical for accurate diagnosis and treatment.

Purpose of the Study:

  • To investigate clonal relationships between primary B-cell lymphomas and their relapsed counterparts.
  • To evaluate the reliability of different molecular methods for assessing clonal origin in lymphoma relapse.

Main Methods:

  • Analysis of Ig gene rearrangements in 27 paired primary and relapsed lymphoma tissues using BIOMED-2 multiplex PCR.
  • Cloning and sequencing of amplified Ig genes in 17 patients to confirm VDJ rearrangements.
  • Comparison of fragment lengths and DNA sequences to establish clonal identity.

Main Results:

  • All 27 cases showed monoclonal Ig gene rearrangements by PCR.
  • Fragment length analysis was discordant in 70% of cases, while sequencing confirmed identical VDJ rearrangements in 77% of cases with differing fragment lengths.
  • Unrelated relapses occurred in a minority of cases, were associated with longer disease-free intervals, and tended towards poorer outcomes.

Conclusions:

  • Relapse from an unrelated clone is an uncommon but significant event in B-cell lymphomas.
  • Sequencing of Ig gene rearrangements provides a more definitive assessment of clonal relationships than PCR fragment size alone.
  • Accurate determination of clonal origin is essential for understanding lymphoma behavior and patient outcomes.

Keywords:
BIOMED-2 multiplex polymerase chain reactionclonalityimmunoglobulin gene rearrangementsrecurrent B-cell lymphomas

More Related Videos

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation09:02

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Published on: November 26, 2018

22.7K
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma10:52

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Published on: March 30, 2018

11.8K

Related Concept Videos

Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
10.0K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
17.2K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
18.0K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
2.0K
Primary Lymphoid Organs01:16

Primary Lymphoid Organs

Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
16.6K
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.6K