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Detection of anti-dsDNA antibodies by computer-aided automated immunofluorescence analysis

Gabriella Lakos1, Marlene Gonzalez1, David Flaherty1

  • 1Inova Diagnostics, Inc, San Diego, CA, USA.

Journal of Immunological Methods
|February 28, 2016
PubMed

Insights

The NOVA View system accurately measures anti-dsDNA antibodies using Crithidia luciliae indirect immunofluorescence test (CLIFT) technology, showing high consistency and agreement with manual readings for diagnostic immunology.

Area of Science:

  • Immunology
  • Diagnostic Technology
  • Microscopy

Background:

  • Indirect immunofluorescent tests are crucial in diagnostic immunology.
  • Automated systems aim to improve the efficiency and reliability of these tests.
  • Anti-dsDNA antibodies are important biomarkers, particularly in systemic lupus erythematosus (SLE).

Purpose of the Study:

  • To evaluate the performance of the NOVA View system for automated anti-dsDNA antibody measurement.
  • To assess the NOVA View system's accuracy using Crithidia luciliae indirect immunofluorescence test (CLIFT) technology.
  • To compare automated results with manual microscopic readings.

Main Methods:

  • Analytical performance was assessed for repeatability and reproducibility.
  • Two hundred fifty patient samples were tested, including 50 SLE patients.
  • Agreement between NOVA View CLIFT and manual microscopic reading was evaluated.

Main Results:

  • NOVA View demonstrated high repeatability and reproducibility across runs and instruments.
  • Agreement between NOVA View digital image reading and manual reading was 96.0%.
  • Positivity rates for anti-dsDNA antibodies in SLE patients were comparable between automated and manual methods.

Conclusions:

  • NOVA View CLIFT results are equivalent to manual microscopic readings in routine settings.
  • The automated system offers reliability and consistency for anti-dsDNA antibody testing.
  • NOVA View is a suitable alternative for automated CLIFT in clinical laboratories.
Abstract

Keywords:
AutoantibodiesCrithidia luciliaeIndirect immunofluorescenceSystemic lupus erythematosusdsDNA

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