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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
The basics of immunological tests
C K Ogle1, J D Ogle, J W Alexander
1Shriners Burns Institute, Cincinnati, Ohio.
Insights
This review covers immune system cells and factors, detailing tests for immune function assessment. It specifies blood volume and assay time for each diagnostic test.
Area of Science:
- Immunology
- Clinical Diagnostics
Background:
- The immune system comprises cellular and humoral components crucial for host defense.
- Understanding immune function is vital for diagnosing and managing various diseases.
Purpose of the Study:
- To provide a comprehensive overview of the cellular and humoral elements of the immune system.
- To detail available diagnostic tests for assessing patient immune functions.
Main Methods:
- Literature review of immunological components.
- Compilation and description of established immune function assays.
Main Results:
- Detailed description of key immune cells (e.g., lymphocytes, phagocytes) and humoral factors (e.g., antibodies, cytokines).
- Presentation of various immune function tests, including their methodologies.
- Information on the blood volume requirements and assay turnaround times for each test.
Conclusions:
- This review consolidates essential information on immune system components and functional testing.
- It serves as a practical guide for clinicians and researchers regarding immune diagnostics.
Abstract:
This review article describes the cells and the humoral factors of the immune system. It also explains what tests are available to assess the immune functions of patients. Each test listed states the amount of blood needed and the time required to perform the assays.
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