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Updated: Mar 24, 2026
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
A sandwich-type electrochemical immunosensor based on the biotin- streptavidin-biotin structure for detection of
Yueyun Li1,2, Yihe Zhang1, Liping Jiang2
1Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences, Beijing, 100083, P. R. China.
Insights
This study presents a novel immunosensor for detecting human immunoglobulin G (HIgG). The device utilizes a functionalized graphene platform and magnetic nanoparticles, achieving high sensitivity and a low detection limit for HIgG.
Area of Science:
- * Biomedical Engineering
- * Biosensor Technology
- * Immunodiagnostics
Background:
- * Accurate detection of human immunoglobulin G (HIgG) is crucial for diagnosing various diseases.
- * Existing detection methods often face limitations in sensitivity, speed, or complexity.
- * Development of advanced immunosensors is needed for improved biomolecule detection.
Purpose of the Study:
- * To design and fabricate a novel sandwich-type immunosensor for sensitive HIgG detection.
- * To utilize a composite material (GS-SnO2-PAN) as the sensing platform.
- * To employ biotin-functionalized magnetic nanoparticles (B-Fe3O4@APTES) for signal amplification and enhanced detection.
Main Methods:
- * Fabrication of a graphene-based sensing platform functionalized with polyaniline and tin dioxide (GS-SnO2-PAN).
- * Utilized biotin-functionalized amination magnetic nanoparticles composite (B-Fe3O4@APTES) as a label for antibody capture.
- * Employed a sandwich assay format with primary anti-HIgG (Ab1) and secondary antibody (Ab2), leveraging the biotin-streptavidin interaction for signal amplification.
Main Results:
- * The developed immunosensor demonstrated a wide sensitivity range for HIgG detection, from 1 pg/L to 10 ng/L.
- * Achieved a low limit of detection (LOD) of 0.33 pg/L (S/N = 3) for HIgG.
- * The immunosensor exhibited high sensitivity, rapid assay time, and excellent reproducibility, specificity, and stability, particularly in serum samples.
Conclusions:
- * The novel sandwich-type immunosensor based on GS-SnO2-PAN and B-Fe3O4@APTES offers a highly sensitive and efficient platform for HIgG detection.
- * The combination of advanced materials and a signal amplification strategy significantly enhances detection capabilities.
- * This immunosensor shows great potential for the quantitative detection of biomolecules in complex biological samples like serum.
Abstract:
A sandwich-type immunosensor is designed and fabricated to detect the human immunoglobulin G (HIgG) using polyaniline and tin dioxide functionalized graphene (GS-SnO2-PAN) as the platform and biotin-functionalized amination magnetic nanoparticles composite (B-Fe3O4@APTES) as the label. GS-SnO2-PAN is used as the sensing agent to capture the primary anti-HIgG (Ab1) and SnO2 reduces the stack of GS. The B-Fe3O4@APTES with a large surface area and excellent biocompatibility captures second antibody (Ab2) efficiently based on the highly selective recognition of streptavidin to biotinylated antibody. The B-Fe3O4@APTES has better electro-catalytic activity in the reduction of hydrogen peroxide (H2O2) and the "biotin-streptavidin-biotin" (B-SA-B) strategy leads to signal amplification. Under optimal conditions, the immunosensor has a wide sensitivity range from 1 pg/L to 10 ng/L and low detection limit of 0.33 pg/L (S/N = 3) for HIgG. The immunosensor has high sensitivity, fast assay rate, as well as good reproducibility, specificity, and stability especially in the quantitative detection of biomolecules in serum samples.
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