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Updated: Mar 24, 2026

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Ly6C(+) monocyte efferocytosis and cross-presentation of cell-associated antigens

S R Larson1,2, S M Atif1, S L Gibbings1

  • 1Department of Pediatrics, National Jewish Health, Denver, CO, USA.

Cell Death and Differentiation
|March 19, 2016
PubMed

Insights

Ly6C(+) monocytes can engulf dying cells and present antigens to CD8(+) T cells, crucial functions in adaptive immunity. Efferocytosis is boosted by TLR4 and TLR7, while cross-presentation is enhanced only by TLR7.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Circulating Ly6C(+) monocytes migrate to lymph nodes with minimal phenotypic changes.
  • Ly6C(+) monocytes are abundant in lymph nodes, especially during inflammation.
  • The functional roles of these constitutively trafficking monocytes are largely unknown.

Purpose of the Study:

  • To investigate the efferocytosis and cross-presentation capabilities of Ly6C(+) monocytes.
  • To determine if these functions are comparable to those of Batf3(+) dendritic cells (DCs).
  • To explore the impact of Toll-like receptor (TLR) activation on these functions.

Main Methods:

  • Assessing efferocytosis and antigen cross-presentation by Ly6C(+) monocytes.
  • Utilizing TLR4 and TLR7 agonists for monocyte activation.
  • Analyzing T cell responses to antigen-presenting monocytes.

Main Results:

  • Ly6C(+) monocytes intrinsically possess efferocytosis and cross-presentation abilities.
  • Efferocytosis by Ly6C(+) monocytes is enhanced by both TLR4 and TLR7 activation.
  • TLR7 activation, but not TLR4, enhances cross-presentation by Ly6C(+) monocytes.
  • These monocytes present antigens to CD8(+) T cells.

Conclusions:

  • Ly6C(+) monocytes exhibit significant functional roles in adaptive immune responses.
  • These monocytes can effectively clear cellular debris and prime T cell immunity.
  • TLR signaling differentially modulates efferocytosis and cross-presentation in Ly6C(+) monocytes.

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