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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Highly sensitive covalently functionalized light-addressable potentiometric sensor for determination of biomarker
Jintao Liang1, Mingyuan Guan2, Guoyin Huang2
1School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; Guangxi Experiment Center of Information Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
Insights
We developed a novel light-addressable potentiometric sensor (LAPS) to detect human immunoglobulin G (hIgG), a key disease biomarker. This sensor shows promise for early disease prediction with simple, reproducible detection.
Area of Science:
- Biosensors
- Biomarker Detection
- Immunoglobulin Assays
Background:
- Biomarkers indicate biological status and aid in early disease prediction.
- Human immunoglobulin G (hIgG) serves as a model biomarker for disease detection.
Purpose of the Study:
- To develop and validate a novel structured light-addressable potentiometric sensor (LAPS) for quantifying human immunoglobulin G (hIgG).
- To assess the sensor's performance, stability, and reproducibility for potential clinical applications.
Main Methods:
- Covalently immobilizing goat anti-human immunoglobulin G antibodies onto a LAPS chip as the recognition element.
- Utilizing a laser diode controlled by a field-programmable gate array to drive the LAPS system.
- Monitoring potential shifts in response to varying concentrations of hIgG in a supporting electrolyte solution.
Main Results:
- Achieved a linear correlation between potential shift and hIgG concentration (ΔV (V)=0.00714ChIgG (μg/mL)-0.0147, R²=0.9968) over a 0-150 μg/mL range.
- Demonstrated acceptable stability and reproducibility of the LAPS system.
- Validated the sensor's capability for sensitive and specific hIgG detection.
Conclusions:
- The developed LAPS system offers a sensitive, stable, and reproducible method for detecting disease biomarkers like hIgG.
- The system's simple operation and multi-sample format make it suitable for environmental, food, and clinical diagnostics.
- This technology holds significant promise for early disease prediction and monitoring.
Abstract:
A biomarker is related to the biological status of a living organism and shows great promise for the early prediction of a related disease. Herein we presented a novel structured light-addressable potentiometric sensor (LAPS) for the determination of a model biomarker, human immunoglobulin G (hIgG). In this system, the goat anti-human immunoglobulin G antibody was used as recognition element and covalently immobilized on the surface of light-addressable potentiometric sensor chip to capture human immunoglobulin G. Due to the light addressable capability of light-addressable potentiometric sensor, human immunoglobulin G dissolved in the supporting electrolyte solution can be detected by monitoring the potential shifts of the sensor. In order to produce a stable photocurrent, the laser diode controlled by field-programmable gate array was used as the light emitter to drive the light-addressable potentiometric sensor. A linear correlation between the potential shift response and the concentration of human immunoglobulin G was achieved and the corresponding regression equation was ΔV (V)=0.00714ChIgG (μg/mL)-0.0147 with a correlation coefficient of 0.9968 over a range 0-150 μg/mL. Moreover, the light-addressable potentiometric sensor system also showed acceptable stability and reproducibility. All the results demonstrated that the system was more applicable to detection of disease biomarkers with simple operation, multiple-sample format and might hold great promise in various environmental, food, and clinical applications.
