Related Experiment Video

Updated: Mar 20, 2026

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
11:31

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Published on: May 30, 2017

10.5K

Immunohisto- and Cytochemistry Analysis of Connexins

Bruno Cogliati1, Michaël Maes2, Isabel Veloso Alves Pereira3

  • 1Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP, Brazil. bcogliati@usp.br.

Methods in Molecular Biology (Clifton, N.J.)
|May 22, 2016
PubMed

Insights

This chapter details a fluorescent immunohistochemistry (IHC) protocol for detecting connexin proteins in various biological samples. This cost-effective method visualizes protein expression and subcellular localization, crucial for understanding protein function.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Histology

Background:

  • Immunohistochemistry (IHC) is vital for analyzing protein expression within tissue morphology.
  • In connexin research, IHC determines subcellular localization, directly correlating with protein function.

Purpose of the Study:

  • To describe a protocol for fluorescent immunohistochemistry (IHC).
  • To detect connexin proteins in tissue slices and cells.
  • To provide modifications based on sample type and protein expression levels.

Main Methods:

  • Fluorescent immunohistochemistry (IHC) protocol.
  • Antibody-antigen recognition utilizing fluorescent labels.
  • Adaptations for diverse biological samples and processing.

Main Results:

  • Successful visualization of connexin proteins in tissue and cell samples.
  • Demonstration of protein subcellular localization.
  • Protocol's simplicity, cost-effectiveness, and speed.

Conclusions:

  • Fluorescent IHC is a versatile and efficient technique for studying connexin proteins.
  • The described protocol allows for precise protein localization and functional inference.
  • This method is adaptable for various research needs in cell and tissue analysis.

Keywords:
AntibodyConnexinsFluorophoresImmunocytochemistryImmunofluorescenceImmunohistochemistryLocalizationMicroscopyMorphology

More Related Videos

Single-cell Microinjection for Cell Communication Analysis09:59

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

11.9K
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

13.5K

Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
14.7K
Gap Junctions01:37

Gap Junctions

Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
58.2K
Gap Junctions01:27

Gap Junctions

The cytoplasm of adjacent animal cells can exchange small molecules, ions, and secondary messengers via the communication channels which form the gap junctions. These junctions comprise a few hundred to thousands of molecular channels, each made of two halves, called the connexon hemichannel. A connexon is a hexamer of six transmembrane connexin proteins, which assemble radially, thus forming a pore or channel in the center. One connexon hemichannel docks with a corresponding connexon on the...
10.5K