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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
Large B-cell lymphoma in a dog: A cyto-histopathological evaluation and Immunophenotyping according to WHO
Zahra Nikousefat1, Mohammad Hashemnia2, Moosa Javdani3
1Department of Clinical Sciences, School of Veterinary Medicine, Razi University, Kermanshah, Iran;
Insights
This study details the diagnosis of diffuse large B-cell lymphoma (DLBCL) in a dog using the World Health Organization classification system. The findings highlight the system
Area of Science:
- Veterinary Pathology
- Canine Oncology
- Immunophenotyping
Background:
- Canine lymphomas represent a significant group of hematopoietic neoplasms in veterinary medicine.
- Accurate classification is crucial for prognosis and treatment strategies.
- The World Health Organization (WHO) system provides a standardized approach to classifying canine lymphomas.
Observation:
- Cyto-histopathological examination of lymph nodes revealed neoplastic cells consistent with lymphoma.
- Fine-needle aspiration showed intermediate to large neoplastic cells with specific cytoplasmic and nuclear features.
- Histopathology confirmed lymph node replacement by sheets of immunoblasts and centroblasts, with high mitotic activity.
Findings:
- Immunohistochemical analysis demonstrated that neoplastic cells express B-cell markers (CD20, CD79a) but not T-cell markers (CD3).
- The case was diagnosed as diffuse large B-cell lymphoma, high-grade centroblastic type (DLBCL-CB) based on integrated diagnostic findings.
- The WHO classification system proved highly useful and consistent for diagnosing canine lymphoma.
Implications:
- This case underscores the utility of the WHO classification system in veterinary lymphoma diagnosis.
- Accurate diagnosis of DLBCL-CB is essential for guiding therapeutic decisions in canine patients.
- Further collaborative studies are recommended to enhance the effectiveness of the WHO classification system for canine lymphomas.
Abstract:
In the present study, we described cyto-histopathological features and immunophenotyping of the large B-cell lymphoma in an 8-year-old mixed breed dog with applying the World Health Organization (WHO) system of classification of canine lymphomas. In fine-needle aspiration (FNA), lymph nodes were involved by neoplastic cells of intermediate to large size with deep blue cytoplasm; consist of centroblasts, immunoblast and medium-sized cells. Histopathologically, the follicles and sinuses of lymph nodes were replaced by sheets of numerous immunoblasts (less than 90.0% of total cells) and centroblasts. Numerous mitotic figures were also observed. Immunohistochemical analysis presented that the neoplastic cells express B-cell phenotype CD20 and CD79a, but do not stain for T phenotype CD3. On the basis of cytology, histopathology and immunohistochemical findings, the present tumor was diagnosed as diffuse large B-cell lymphoma, high-grade centroblastic type (DLBCL-CB) according to WHO histological classification. Applying this classification system for diagnosis of canine lymphomas is very useful and has a high accuracy and consistency. However, further co-operative studies between clinicians and pathologists should be performed, in order to improve the effectiveness of this classification.
