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Updated: Mar 20, 2026
Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues
Published on: October 26, 2021
Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues
Chun Wei Chiam1, I-Ching Sam1, Yoke Fun Chan1
1Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Insights
This study presents a new immunohistochemistry method to detect Chikungunya virus (CHIKV) antigens in mouse brains. The technique enables precise visualization of viral proteins within infected tissues.
Area of Science:
- Neuroscience
- Immunology
- Virology
Background:
- Immunohistochemistry is a key technique for detecting proteins in tissues.
- Chikungunya virus (CHIKV) poses a significant public health threat, necessitating robust diagnostic methods.
Purpose of the Study:
- To develop and describe a novel immunohistochemistry protocol for localizing CHIKV antigens.
- To validate the method's efficacy in infected mouse brain tissue.
Main Methods:
- Utilizing formalin-fixed, paraffin-embedded mouse brain samples.
- Employing specific antibody binding to target CHIKV antigens.
- Visualizing antigen-antibody complexes via a chromogenic substrate reaction.
Main Results:
- Successfully localized Chikungunya virus antigens within infected mouse brain tissue.
- Demonstrated the specificity and sensitivity of the developed immunohistochemistry method.
Conclusions:
- The described immunohistochemistry method provides a reliable tool for detecting CHIKV in neural tissues.
- This technique can aid in understanding CHIKV neuropathogenesis and evaluating antiviral strategies.
Abstract:
Immunohistochemistry is a histological technique that allows detection of one or more proteins of interest within a cell using specific antibody binding, followed by microscopic visualization of a chromogenic substrate catalyzed by peroxidase and/or alkaline phosphatase. Here, we describe a method to localize Chikungunya virus (CHIKV) antigens in formalin-fixed and paraffin-embedded infected mouse brain.
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