Immunocytochemical Analysis of Lymphoid Lesions Using -Fine Needle Aspiration Biopsy
D C Brown1, K C Gatter1, D Y Mason1
1a Nuffield Department of Pathology, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK.
Insights
Fine needle aspiration (FNA) biopsy is valuable for diagnosing lymphoid lesions. Combining conventional cytology with immunocytochemistry improves diagnostic accuracy for conditions like lymphoma and carcinoma.
Area of Science:
- Oncology
- Pathology
- Cytology
Background:
- Lymphoid lesions require accurate diagnosis for effective treatment.
- Fine needle aspiration (FNA) is a minimally invasive diagnostic technique.
- Immunocytochemistry aids in differentiating cell types and disease states.
Purpose of the Study:
- To evaluate the diagnostic utility of FNA in investigating lymphoid lesions.
- To assess the combined efficacy of conventional cytology and immunocytochemistry.
- To determine the concordance between FNA diagnoses and histological findings.
Main Methods:
- Investigated 64 cases of lymphoid lesions using FNA.
- Prepared both conventional smears and cytospin slides.
- Utilized cytological and immunocytological criteria for diagnosis.
- Confirmed diagnoses with tissue sections and clinical information.
Main Results:
- Diagnostic criteria were applied to 57 cases.
- Diagnoses included reactive hyperplasia, lymphomas, and metastatic carcinoma.
- Histological confirmation supported 48 out of 51 FNA diagnoses.
- Only 3 diagnoses required significant alteration after histology.
Conclusions:
- FNA biopsy is an effective method for evaluating lymphoid lesions.
- The combination of conventional cytology and immunocytochemistry enhances diagnostic precision.
- FNA provides reliable preliminary diagnoses for lymphoid pathologies.
Abstract:
Sixty-four cases of mainly lymphoid lesions were investigated by fine needle aspiration. Both conventional smears and cytospin preparations were made from this material. Diagnoses based on cytological and immunocytological criteria were made in 57 of these cases and include reactive hyperplasia, non-Hodgkin's and Hodgkin's lymphoma and metastatic carcinoma. Five cases were inadequate for diagnostic purposes and in 2 cases no definite diagnosis could be made. These diagnoses were confirmed using tissue sections in 48/51 cases (with only 3 cytological diagnoses significantly altered by histology) and supported by supplementary clinical information in 8 cases. These results demonstrate the value of FNA biopsy in the investigation of lymphoid lesions using both conventional cytology and immunocytochemistry.
