Related Experiment Video
Updated: Mar 17, 2026
In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)
Published on: August 1, 2025
A Novel Cell Line (NCU-L-1) from a Patient with Acute Lymphoblastic Leukemia, FAB, L3, Burkitt's Type
1a The Second Department of Internal Medicine, Nagoya City University Medical School Nagoya, Japan.
Insights
A new cell line, NCU-L-1, derived from Burkitt
Area of Science:
- Hematology
- Oncology
- Cell Biology
Background:
- Acute lymphoblastic leukemia (ALL), specifically L3 FAB (Burkitt's type), is a significant hematologic malignancy.
- Characterization of novel cell lines is crucial for understanding leukemia pathogenesis and developing targeted therapies.
Purpose of the Study:
- To establish and characterize a novel human cell line, NCU-L-1, from a patient with Burkitt's type ALL.
- To investigate the immunophenotype and cytogenetic profile of the NCU-L-1 cell line for potential research applications.
Main Methods:
- Establishment of a cultured cell line (NCU-L-1) from patient peripheral blood.
- Immunophenotypic analysis using surface marker detection (IgG Lambda, CD19, CD20, la-like antigen).
- Cytogenetic analysis to identify chromosomal abnormalities.
Main Results:
- NCU-L-1 cells exhibited a mature B-cell phenotype with specific surface markers.
- Cytogenetic analysis revealed a novel translocation t(2; 8) and an additional 14q+.
- IgG was detected in the cell culture supernatant, with no intracytoplasmic immunoglobulin.
Conclusions:
- The NCU-L-1 cell line represents a unique model for studying mature B-cell malignancies.
- The novel t(2; 8) translocation and 14q+ abnormality in NCU-L-1 offer opportunities to study specific oncogenic events.
- This cell line is valuable for research into karyotype evolution and therapeutic strategies in Burkitt's lymphoma.
Abstract:
A novel cultured cell line, NCU-L-1, was established from a 71-year-old Japanese woman with acute lymphoblastic leukemia, L3 FAB, Burkitt's type. The NCU-L-1 cells were shown to have a mature B-cell phenotype on the basis of immunologic surface marker analysis; including IgG Lambda surface immunoglobulins, CD19, CD20 and la-like antigen which were all detected on the cells. Intracytoplasmic immunoglobulin was not detected, but IgG was present in the cell culture supernatant. Cytogenetic studies revealed that the NCU-L-1 cells had t(2; 8) and an additional 14q+, a genotype which has not been identified previously in the usual Burkitt's cell lines. The NCU-L-1 cell line should prove to be useful for studying oncogenic events associated with the t(2; 8) translocation and karyotype evolution.
More Related Videos
09:43An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
Published on: March 30, 2018
09:09Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
Published on: November 10, 2017
