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Bone marrow B lymphocytes in multiple myeloma and MGUS: Focus on distribution of naïve cells and memory subsets

Fanny Pojero1, Alessandra Casuccio2, Caterina Giambanco3

  • 1Dipartimento di Biopatologia e Biotecnologie Mediche, Universita' degli Studi di Palermo, Corso Tukory 211, 90134 Palermo, Italy; U.O.S.D. Laboratorio Specialistico Oncologia, Ematologia e Colture Cellulari per Uso Clinico, ARNAS Civico, Piazza Nicola Leotta 4, 90127 Palermo, Italy.

Leukemia Research
|August 24, 2016
PubMed

Insights

Multiple myeloma impacts B cell populations in bone marrow. Specific B cell subsets may serve as predictive markers for treatment response in patients with multiple myeloma (MM).

Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • Multiple myeloma (MM) is characterized by abnormal plasma cell proliferation in the bone marrow (BM).
  • MM is associated with immune system defects, particularly affecting B cell compartments, influencing disease onset and progression.

Purpose of the Study:

  • To investigate the distribution of distinct B lymphocyte subsets in the BM of healthy controls and multiple myeloma patients.
  • To explore the potential of specific B cell populations as predictive markers in MM.

Main Methods:

  • Flow cytometry was employed to analyze B cell subsets: naïve/transitional (IgD(+)CD27(-)), memory unswitched (IgD(+)CD27(+)), memory switched (IgD(-)CD27(+)), and double negative (DN) (IgD(-)CD27(-)).
  • B cell distribution was compared between healthy controls, responding MM patients, and relapsing MM patients.

Main Results:

  • Healthy controls showed a higher percentage of IgD(+)CD27(+) B cells compared to responding patients.
  • Treated patients with incomplete response had an expanded DN B cell compartment compared to complete responders.
  • Stringent complete responders had a larger IgD(+)CD27(-) B cell subpopulation than other responding patients.
  • No clonal restriction was observed in any studied B cell subset.

Conclusions:

  • B cell subset alterations in the MM bone marrow microenvironment warrant further investigation, potentially linking to immunosenescence and infection risk.
  • Naïve/transitional and DN B cells may serve as predictive biomarkers in treated multiple myeloma patients.
Abstract

Keywords:
B cellsFlow cytometryMGUSMultiple myeloma

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