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Updated: Mar 13, 2026

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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Clonal Proliferation and Stochastic Pruning Orchestrate Lymph Node Vasculature Remodeling

Isabelle Mondor1, Audrey Jorquera1, Cynthia Sene1

  • 1Aix-Marseille Université, CNRS, INSERM, CIML, 13288 Marseille, France.

Immunity
|October 21, 2016
PubMed

Insights

Lymph node vasculature expands via sequential assembly of endothelial cell units, driven by high endothelial venule cells. Vascular contraction involves cell death, revealing immune response dynamics.

Area of Science:

  • Immunology
  • Vascular Biology
  • Cell Biology

Background:

  • Lymph node (LN) expansion during immune responses involves vascular remodeling.
  • Mechanisms of LN endothelial cell division are partially understood, but single-cell dynamics remain unclear.

Purpose of the Study:

  • To comprehensively map LN endothelial cell dynamics at the single-cell level during immune responses.
  • To elucidate the mechanisms of vascular expansion and contraction in lymph nodes.

Main Methods:

  • Utilized multicolored fluorescent fate-mapping models.
  • Tracked blood endothelial cell behavior during LN expansion and return to homeostasis.

Main Results:

  • LN vascular expansion occurs through sequential assembly of endothelial cell proliferative units.
  • High endothelial venule (HEV) cells act as progenitors, driving clonal proliferation for new vessels.
  • Vascular contraction involves stochastic death of both existing and newly formed endothelial cells.

Conclusions:

  • Single-cell fate mapping reveals complex dynamics of vascular remodeling in lymph nodes.
  • Identified HEV cells as key progenitors in LN vascular expansion.
  • Demonstrated the role of cell death in LN vascular homeostasis following immune response.

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