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Updated: Mar 5, 2026
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
Published on: March 25, 2020
Phenotyping Multiple Subsets of Immune Cells In Situ in Formalin-Fixed, Paraffin-Embedded Tissue Sections
James R Mansfield1, Paul C Guest2, Jared Burks3
1Andor Technology, 5 Millennium Way, Belfast, BT12 7AL, UK. jim@jmansfield.com.
Insights
Psychiatric symptoms can signal somatic illnesses like peripheral tumors, often linked to inflammation. A new multispectral imaging protocol analyzes immune cells and protein expression in tumors to understand these connections.
Area of Science:
- Oncology
- Immunology
- Biomedical Imaging
Background:
- Somatic illnesses, including peripheral tumors, can manifest with psychiatric symptoms.
- Biomarker changes related to inflammation and immune response are often observed in these cases.
Purpose of the Study:
- To introduce a novel multispectral imaging protocol.
- To enable simultaneous imaging of multiple proteins in tissue sections for cell phenotyping.
Main Methods:
- Utilizing a multispectral imaging protocol for tissue sections.
- Simultaneous imaging of multiple proteins to phenotype immune and other cell types.
- Assessing the spatial organization of cells within peripheral solid tumors and other tissues.
Main Results:
- The protocol allows for detailed phenotyping of various cell types within tumor microenvironments.
- It enables the assessment of immune cell distribution and interactions.
- Spatial organization of cells can be quantitatively analyzed.
Conclusions:
- Multispectral imaging offers a powerful approach to study the interplay between somatic illnesses, psychiatric symptoms, and the immune system.
- This method aids in understanding the tumor microenvironment and potential therapeutic targets.
Abstract:
Some somatic illnesses such as peripheral tumours can present with psychiatric symptoms. Many of these are characterized by changes in biomarkers related to the inflammation or immune response. Here, we describe a multispectral imaging protocol that can be used to phenotype immune and other cell types through simultaneous imaging of multiple proteins in sections of peripheral solid tumours and other tissues. This approach can also be used to assess the spatial organization of these cells within the tissue.
