Related Experiment Video
Updated: Aug 8, 2026
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
One-step colorimetric detection of an antibody based on protein-induced unfolding of a G-quadruplex switch
Xiaolu Hu1, Chao Li, Chang Feng
1State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, P. R. China. xiangy@nju.edu.cn genxili@nju.edu.cn.
Insights
A novel colorimetric assay enables sensitive antibody detection. This method utilizes protein binding signals and a unique DNAzyme strategy for enhanced specificity.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Molecular Biology
Background:
- Antibody detection is crucial in diagnostics and research.
- Existing methods may lack sensitivity or selectivity.
- Novel assay development is needed for improved antibody quantification.
Purpose of the Study:
- To develop a simple, sensitive, and selective colorimetric assay for antibody detection.
- To integrate protein binding-induced signaling with DNAzyme conformational switching.
Main Methods:
- A novel DNAzyme-based conformational switching strategy was employed.
- A protein binding-induced signaling approach was combined with the DNAzyme strategy.
- The assay was optimized for sensitive and selective colorimetric detection.
Main Results:
- The developed assay demonstrated high sensitivity for antibody detection.
- The assay exhibited excellent selectivity, distinguishing target antibodies effectively.
- The colorimetric readout provided a simple and accessible detection method.
Conclusions:
- This study presents a robust and efficient colorimetric assay for antibody detection.
- The integration of protein binding and DNAzyme strategies offers a promising approach for biosensing.
- The assay has potential applications in clinical diagnostics and biochemical analysis.
Abstract:
A simple colorimetric assay is developed for the sensitive and selective detection of an antibody, which combines a protein binding-induced signaling approach with a novel DNAzyme-based conformational switching strategy.
