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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
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Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse

Xinlong Jiang1, Wenzhong An1, Xiao Yang1

  • 1Laboratory of Animal Cell and Molecular Biology, College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, Liaoning, 110866, China.

Gene Expression Patterns : GEP
|November 21, 2017
PubMed

Insights

The CRUMBS polarity complex proteins are transcribed early in mouse development but only become asymmetrically distributed at the compacted 8-cell stage, suggesting their role in establishing early embryonic cell polarity.

Area of Science:

  • Developmental biology
  • Cell biology
  • Molecular biology

Background:

  • Blastomeres in mouse preimplantation development are equipotent until polarity establishment.
  • Cell polarity involves the asymmetric distribution of polarity proteins along the apical-basal axis.

Purpose of the Study:

  • To investigate the temporal and spatial expression of CRUMBS polarity complex proteins in early mouse embryos.
  • To understand the role of the CRUMBS complex in establishing cell polarity during preimplantation development.

Main Methods:

  • Analysis of gene transcription for Crb3, Pals1, Patj, and Mpdz in mouse preimplantation embryos.
  • Observation of protein localization patterns of CRUMBS complex components from compaction onwards.

Main Results:

  • Crb3, Pals1, Patj, and Mpdz are transcribed in the mouse preimplantation embryo.
  • Asymmetric distribution of these proteins is established by the compacted 8-cell stage.
  • CRB3 and PALS1 localize to the apical membrane; PATJ and MPDZ localize to tight junctions and adjacent apical membrane.

Conclusions:

  • The CRUMBS polarity complex is likely involved in establishing cell polarity in early mouse embryos.
  • Developmental spatial asymmetries are initiated at the compaction stage.
  • This study provides a basis for exploring the CRUMBS complex's function in trophectoderm specification and blastocyst morphogenesis.

Keywords:
CRB3MPDZMouse preimplantation embryoPALS1PATJ

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